FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187087
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39640.3339266624939874No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCCCC36150.3045269639040778No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33840.2850675645508712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30200.25440426860036375No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29460.24817052162141445No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGG28190.23747206396835277No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27030.22770024437972955No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26870.22635240719509186No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26150.22028713986422224No Hit
GAGCACCACTGTGCTTTTCGGCGGAGGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA25730.21674906725454832No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25590.21556970971799033No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24930.21000988133135987No Hit
GTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24720.20824084502652293No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGGAGATACA23830.20074350068697575No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23270.19602607054074386No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22380.1885287262011967No Hit
GATTATTACTGCTGCTCATTTACAAGGAGCACCACTGTGCTTTTCGGCGGAGGGACCAGGGTGACCGTCCTAGGT21260.17909386590873289No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGCACAGTGGTGCTCCTTGTAAATGAGCAGCAGTAATAAT20390.17176500121726546No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20310.17109108262494663No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20250.17058564368070747No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19970.16822692860759153No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19740.16628941265467484No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19080.16072958426804437No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGCACAGTGGTGCTCCTTGTAAATGAGCAG19070.16064534444400452No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18470.15559095500161318No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18100.15247408151213854No Hit
GTGGTGCTCCTTGTAAATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGT17690.14902024872650446No Hit
GTGTATTACTGTGCGACAGAAGGGCCTTGGGATGGTTACGTATTGGACCA17530.14767241154186678No Hit
CCCCTGGGATCCTGCAGCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16940.1427022619235153No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16720.14084898579463848No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16650.14025930702635947No Hit
CGCATGAAGGGGGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC16600.13983810790616022No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15800.13309892198297177No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15720.1324250033906529No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15570.13116140603005508No Hit
CTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15340.12922389007713841No Hit
GCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15270.12863421130885944No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14870.1252646183472652No Hit
GTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14530.12240046432991011No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14520.12231622450587026No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14380.12113686696931228No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAGTGACATT14370.12105262714527242No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14300.12046294837699342No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT14290.12037870855295357No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAG14280.12029446872891371No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATGGTCAGGGAGG14150.11919935101639559No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGGGCA14000.11793575365579775No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13920.11726183506347893No Hit
TCTCTGGGCTCCAGACTGAGGACGAGGCTGATTATTACTGCTGCTCATTT13760.11591399787884123No Hit
GTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG13720.11557703858268181No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGG13640.11490311999036297No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13590.11448192087016369No Hit
CTGCAGCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT13520.1138922421018847No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13460.11338680315764556No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC13030.10976449072393178No Hit
CACCAGTGTGGCCTTGTTGGCTCGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12950.10909057213161293No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC12860.10833241371525422No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12770.10757425529889554No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12460.10496282075366001No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12320.10378346321710202No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12110.10201442691226506No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11960.10075082955166724No Hit
GTCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC11940.10058234990358753No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT11940.10058234990358753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)