FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187087
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37570.3164890189177373No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCCCC36910.3109291905311068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35330.29761929833280965No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33010.27807565915556315No Hit
CCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29940.2522140331753275No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28520.24025197816166804No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27130.2285426426201281No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26160.2203713796882621No Hit
GTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25290.2130425149967947No Hit
GAGCACCACTGTGCTTTTCGGCGGAGGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA25050.21102075921983812No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24870.20950444238712077No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGG24290.20461853259280913No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23770.20023806174273664No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22770.19181407933875105No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTATTCATCTGGAGATACA21750.18322161728668582No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21610.18204225975012783No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20700.17437643576250098No Hit
GATTATTACTGCTGCTCATTTACAAGGAGCACCACTGTGCTTTTCGGCGGAGGGACCAGGGTGACCGTCCTAGGT19650.16553125423831616No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19450.16384645775751905No Hit
GACCTAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGCACAGTGGTGCTCCTTGTAAATGAGCAGCAGTAATAAT19400.16342525863731974No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19330.16283557986904076No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19040.16039262497188497No Hit
GTGGTGCTCCTTGTAAATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGT18660.15719151165837045No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTCCGCCGAAAAGCACAGTGGTGCTCCTTGTAAATGAGCAG18100.15247408151213854No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18050.15205288239193926No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17980.15146320362366025No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17600.14826209031014576No Hit
GTGTATTACTGTGCGACAGAAGGGCCTTGGGATGGTTACGTATTGGACCA17070.14379737963603342No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16700.14068050614655875No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16670.1404277866744392No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16530.1392484291378812No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16010.13486795828780873No Hit
CGCATGAAGGGGGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC15830.13335164145509132No Hit
CCCCTGGGATCCTGCAGCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15690.13217228391853333No Hit
GTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15370.12947660954925796No Hit
CTGCAGCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT15300.128886930780979No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGGGCA15300.128886930780979No Hit
GCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15080.12703365465210215No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15040.12669669535594275No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCGAGCCAACAAGGCCACACTGGTGT14950.12593853693958404No Hit
GTCCTGGGCCCAGTCTGCCCTGGCTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATTTCCTG14820.1248434192270659No Hit
CTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14500.12214774485779054No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14060.11844119260003691No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT13610.11465040051824339No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13580.11439768104612384No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCCCCCTTCATGCGTGACCTGGCAG13380.11271288456532673No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAGTGACATT13340.11237592526916729No Hit
TCTCTGGGCTCCAGACTGAGGACGAGGCTGATTATTACTGCTGCTCATTT13230.1114492872047289No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13140.11069112878837019No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12950.10909057213161293No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCCCCCGTGGTGGGGGGTGAGGGTTGAGAAC12890.1085851331873738No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12350.1040361826892216No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12280.10344650392094261No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12250.10319378444882303No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCTC12080.1017617074401455No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11970.1008350693757071No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGCCCAGAGATGGTCAGGGAGG11930.10049811007954766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.043.2481351
TGGTATC3950.040.952252
GTATCAA35600.038.9117161
GTGGTAT4400.038.300631
TATCAAC41850.032.9352872
ATCAACG43450.031.9551683
TCAACGC43900.031.5495134
CAACGCA44350.031.3066965
AACGCAG44750.031.103486
CGCAGAG45400.030.5839318
ACGCAGA46100.030.1195347
AGAGTAC45950.030.06863411
CAGAGTA46000.030.03468310
GCAGAGT47050.029.5101369
CTTAGAC500.00867258226.9602323
TGCGCAA500.00867614926.9579610
TACGGTC659.932274E-425.92111410
GTCACCG9400.025.6683470
AACTGTA1350.001084053425.5325370
GAGTACG45500.024.59735912
Produced by FastQC (version 0.11.5)