FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111033
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5140.4629254365819171No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.41339061360136176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.36295515747570545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.3260291985265642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.299910837318635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.2557798132086857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.2431709491772716No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2610.2350652508713626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.22155575369484748No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.2053443570830294No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.20264245764772637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.20174182450262537No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.19994055821242335No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.1774247295848982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1960.1765240964397972No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.17562346329469616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.1630145992632821No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.15220700152207No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.151306368376969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.148604468941666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.147703835796565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.146803202651464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.14139940378085794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.14049877063575694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.13959813749065592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1323930723298479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.13149243918474687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12788990660434285No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.12698927345924185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.12338674087883782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.1224861077337368No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1360.1224861077337368No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1215854745886358No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1215854745886358No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1215854745886358No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1206848414435348No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1310.1179829420082318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.11528104257292877No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1270.11438040942782776No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1230.11077787684742374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1230.11077787684742374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10987724370232273No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1071753442670197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)