FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111033
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9930.8943287130852989No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.4296020102131799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.4232975781974728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.36925958949141247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3670.3305323642520692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.30711590247944304No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.21885385425954446No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.2098475228085344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.20714562337323136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.2053443570830294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.2053443570830294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.19633802563201933No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.18643106103590823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.1837291616006052No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.1810272621653022No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.1801266290202012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.17202093071429214No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1910.17202093071429214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1790.16121333297308008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.16031269982797908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1680.151306368376969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.150405735231868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.147703835796565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.13779687120045392No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1520.1368962380553529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.1359956049102519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.1332937054749489No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1323930723298479No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12788990660434285No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.12428737402393883No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1206848414435348No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.11888357515333278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.1179829420082318No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1290.11618167571802979No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1280.11528104257292877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11438040942782776No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.11347977628272676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.10537407797681769No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1150.10357281168661568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.10357281168661568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATC150.00515641456.5847317
TAGACTC150.005212003456.431735
TTAGACT150.005212003456.431734
ACATTGC150.005212003456.431736
TCGACAG250.00645685353.43755354
CTCAGAG256.8952946E-445.145388
TCAACTC357.406225E-540.3083764
TAGTGAC300.001664672437.74020418
CTTGTAT300.001675801637.6890916
CAACTCA401.6254938E-435.269835
ACTCAGA401.6254938E-435.269837
AGTATTC350.003569329632.30493516
GTAGTGA453.194743E-431.43595917
AACTCAG453.24593E-431.3509586
TGATAGG450.006637517428.4530833
ACGGGTA505.9530145E-428.26681516
TCAGAGT400.006933693328.2031529
CATAGCG607.351591E-427.26010135
ACGTTCG600.0097002326.31820344
ATAGGGC500.00998160326.16969735
Produced by FastQC (version 0.11.5)