FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872608
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28630.32809692324617695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27130.31090707396677547No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25910.2969259965528622No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23070.26437988191719536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22840.2617441050276871No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18370.2105183541750706No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18230.20891396824232644No Hit
GGGTAATCGTAATCATGTAGACAGTAATAAGTTGCAACATCTTCAGGCTG17740.20329861747772196No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17700.2028402214969379No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17230.19745406872272547No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16970.1944744948476292No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16880.1934431038908651No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16470.18874454508782867No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16130.1848481792511643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15350.17590945762587554No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14480.16593934504382266No Hit
GATCTATGCTGCATCCATTTTAGAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGCACAGATTTCA14140.1620429792071583No Hit
CGATTACCCTTTGTCGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14010.16055319226961018No Hit
ACTTATTACTGTCTACATGATTACGATTACCCTTTGTCGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA13890.15917800432725804No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13820.158375811360886No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13660.15654222743774981No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13530.1550524405002017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13080.14989548571638123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13030.1493224907404012No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGACCCACTGCCG12570.14405093696138474No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12560.1439363379661887No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12320.14118596208148448No Hit
GTCCTGATCTATGCTGCATCCATTTTAGAAAGTGGGGTCCCATCACGGTT12240.14026917011991638No Hit
GTCTACATGATTACGATTACCCTTTGTCGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCAC12220.14003997212952438No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12160.1393523781583483No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAGTGATTTAGGCTGGTATCAGCAGAAA12060.1382063882063882No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTCTACATGATTACGATTAC11880.13614360629286001No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11830.13557061131687997No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11060.1267464886867872No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10960.1256004987348271No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10880.12468370677325902No Hit
GTCTCCATCCTCCCTGTTTGCATCTGTAGGAGACAGAGTCACCATCACTT10730.12296472184531887No Hit
GCATAGATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC10600.12147493490777073No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10380.1189537570134585No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10290.11792236605669441No Hit
GATCAGGACTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAT10180.1166617771095383No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGAC10100.11574498514797021No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9950.11402600022003007No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9910.11356760423924604No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9850.11288001026806997No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9610.11012963438336572No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9600.11001503538816972No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9480.1086398474458176No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9350.10715006050826946No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9100.1042850856283692No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9010.10325369467160511No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8950.10256610070042906No Hit
GTTCAGCGGCAGTGGGTCTGGCACAGATTTCACTCTCACCATCAGCAGCC8880.10176390773405698No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8840.10130551175327296No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC8830.10119091275807694No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8750.10027412079650887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT100.009956065129.4293270
CGTACAC200.008050567550.58653
GTATCAA37150.037.6169321
CGTTGTC2200.034.82013363
CCGTTGT2200.034.6996562
TATCAAC42100.033.0166322
GTGGTAT4300.032.9702871
ATCAACG43600.031.8679493
GCCGTTG2300.031.50920761
TCAACGC44100.031.508444
CAACGCA44150.031.4727575
TGCCGTT2300.031.40090260
AACGCAG44050.031.3910776
CGCAGAG45850.030.1569848
ACGCAGA45950.030.0930797
CCGGTTT604.370818E-429.82706657
CCCGGTT604.435067E-429.75285156
AGAGTAC46700.029.53587311
CAGAGTA47100.029.42486810
GCAGAGT47650.029.0161279
Produced by FastQC (version 0.11.5)