FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109597
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT10290.9388943128005329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.3987335419765139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.3768351323485132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.36679836126901283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.3175269396060111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.28741662636751003No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.27008038541200946No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.24361980711150852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.2381452047045083No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.2363203372355083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.20529759026250718No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.200735421590007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.19891055412100694No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.18796134930700656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.18613648183800652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2040.18613648183800652No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.18522404810350648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.18248674690000638No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.17244997582050603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.16697537341350585No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG1600.1459893975200051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14507696378550508No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14507696378550508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.143252096316505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.13960236137850487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.13777749390950483No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.1368650601750048No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.13504019270600473No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1341277589715047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.13321532523700466No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12956559029900452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1400.12774072283000446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1380.12591585536100441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.12044125295400422No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.11314178307800396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.11222934934350393No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.11131691560900389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10949204814000384No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10949204814000384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10949204814000384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1085796144055038No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10766718067100377No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1180.10766718067100377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.10766718067100377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1170.10675474693650373No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC1160.1058423132020037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.10310501199850361No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.10219257826400358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1750.0115.7306370
AAGGGGG1550.076.96892569
CTTGAAA2250.066.3019857
GCTTGAA2250.065.4126856
TTGAAAA2500.064.2259258
AAAAGGG1550.063.04162267
GCTTCTG300.00340366162.79043669
TCTGCTT2200.062.43597853
AAAGGGG1650.062.1737668
ACAAAAT200.003708626661.47709346
TGAAAAA2700.060.43955259
CTGCTTG2300.060.32767554
TTCTGCT2300.059.29570452
AAATGGG553.2021853E-759.2209267
TGCTTGA2450.059.14731655
GTCTTCT2450.055.75227749
TATGCCG2300.054.83746743
CCGTCTT2500.054.71750647
CGTATGC2300.054.19772341
TCTTCTG2550.054.12740350
Produced by FastQC (version 0.11.5)