FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109597
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16311.4881794209695522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.4334060238875152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.38322216849001345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.33212587935801163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.3175269396060111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3090.2819420239605099No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.2545690119255089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.2253711324215079No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2460.22445869868700785No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.22172139748350778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.2171592288110076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.21350949387300747No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.19799812038650694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.19617325291750687No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.17792457822750624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.1770121444930062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1860.16971267461700593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.16697537341350585No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1830.16697537341350585No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.16423807221000575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1505515661925053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.14872669872350522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14507696378550508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.14416453005100505No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.13868992764400487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.12956559029900452No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT1370.12500342162650438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.12226612042300428No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.12135368668850426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.11952881921950419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11587908428150405No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1210.11040448187450386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.11040448187450386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.10949204814000384No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1085796144055038No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.10675474693650373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1100.1003677107950035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTTG100.003737759179.3744770
AGGTCCA200.002876573265.5454356
AGAGCAG200.003895797860.71974648
CCGGGTG450.00412206259.7914970
TCGGAAG200.00423240859.46347846
AGTCTAA250.004693086757.9201563
ATGCTCT200.00472575757.8318643
CTCATGC200.007106809452.1695538
CGGCATA300.001414269239.01249325
TATAAAA950.001644419437.76304670
GAATTAA500.002570297334.52805355
CGGGAAC350.0032102133.0115517
GGGAAGT505.244725E-428.88510918
CAGGTAT657.7577715E-526.6511
ATACCAT600.001528668824.0489527
ACGGGTA752.0433868E-423.10808616
AAGTGGT700.003693404420.66053421
GGAAGTG700.003713047420.6416519
ACGGGGC700.00372290120.6322216
GTATCAA28100.020.3439121
Produced by FastQC (version 0.11.5)