FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964676
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46220.47912459727411066No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTACCAGTATCCCACAC42440.4399404566921951No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACG38410.39816477242099935No Hit
GGATTACCTGTGCGGGAAATAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC34280.3553524706740916No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27310.28310023261696154No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27120.2811306594131086No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC25420.2635081623260037No Hit
CCCCTGTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCTCT24540.2543859285397377No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23130.23976962213219777No Hit
GTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC22450.2327206232973558No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21510.2229764190256625No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCAAAGAGCTCAGTGTCAGGGA21150.2192445961130991No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT21020.21789699339467344No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19740.20462828970555916No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19690.20410998096770314No Hit
CTATTATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA19620.20338434873470473No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18600.19281085048244176No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT18570.19249986523972817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18550.19229254174458574No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17950.18607283689031343No Hit
CCCCAGGAGAGACGGCCAGGATTACCTGTGCGGGAAATAACATTGGAAGT17220.17850552931761543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17160.17788355883218823No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16900.1751883533953369No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC16780.17394441242448241No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGGATTAC16480.17083455999734626No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16210.16803569281292371No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16190.1678283693177813No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16130.16720639883235408No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16120.16710273708478288No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC16080.16668809009449803No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15730.16305992892950585No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15690.16264528193922104No Hit
GTATTATTGTGCGCGAGAGAGTGGGAAGCAGTGGCCACCTCAACAATATGACTCCTGGGGCCAGGGAACCCTGGT15580.1615050027159378No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15440.1600537382499409No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15430.15995007650236973No Hit
ATGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGA15200.157565856308232No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG15140.15694388582280475No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATACTGGTA14850.1539376951432398No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA14600.15134615145395966No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14540.15072418096853243No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14240.14761432854139628No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTA14170.14688869630839785No Hit
GTTTTACTTCCAATGTTATTTCCCGCACAGGTAATCCTGGCCGTCTCTCCTGGGGCCACTGACACCGAGGGTGG13970.14481546135697373No Hit
TGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC13410.13901040349298624No Hit
CTATTACTGTCAGGTGTGGGATACTGGTACTGATCATGTGTTATTCGGCG13210.13693716854156215No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCCCTCAGAGATCCCTGAGGGCCGGTCGCTATTATCATAGA13190.13672984504641972No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13130.13610787456099252No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGG12980.13455294834742443No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC12960.13434562485228202No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12920.13393097786199717No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12890.13361999261928356No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTAC12770.1323760516484291No Hit
GTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGG12700.13165041941543068No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT12640.13102844893000343No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12580.1304064784445762No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12520.12978450795914898No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12080.12522339106601593No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11880.12315015611459185No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11820.12252818562916461No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11820.12252818562916461No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT11660.12086959766802532No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGG11500.11921100970688604No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG11490.11910734795931484No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC11480.11900368621174362No Hit
GCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGG11460.11879636271660123No Hit
GATCATGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11390.11807073048360278No Hit
ATACTGGTACTGATCATGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT11350.11765608349331795No Hit
ATCTCTGGGGGCGCCGTCAGTAGTGGTGGTTCCTACTGGAGCTGGATCCG11300.11713777475546194No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAG11190.11599749553217868No Hit
CCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGGATTACCTGTGCGGGAAATAACATTGGAAGTAAAACTGTG11150.11558284854189385No Hit
CACATGATCAGTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGT10980.11382059883318336No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10910.11309496660018493No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC10650.1103997611633336No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10500.1088448349497655No Hit
GTCCTGGACCTCCTGTGCAAGATCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC10470.1085338497070519No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10400.10780821747405347No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10370.10749723223133983No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10300.1067715999983414No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10250.10625329126048538No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10190.10563132077505816No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10090.1045947032993461No Hit
GAATAACACATGATCAGTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTG10080.1044910415517749No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10020.10386907106634767No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9960.10324710058092043No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9840.10200315961006598No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9740.10096654213435391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1300.047.8648241
TCGTATG1300.047.82470740
CGCTAAT1250.046.64225432
TCGCTAA1300.044.8226831
GGTATCA8300.043.6631851
ACTCGCT1350.043.10433629
AATCTCG1450.042.7273936
GCGGCAT806.5115666E-541.2335970
GCTAATC1500.041.18277733
CTCGCTA1450.040.14829330
TCTCGTA1650.039.7027138
AGGGGGG2200.038.9844870
ACACTCG1500.038.76774227
GTATCAA20050.037.6847421
TATGCCG1700.036.68519643
ATCTCGT1700.036.470637
CACGAGA1700.036.19271521
GTATGCC2150.035.41380742
AACGAAC501.7182155E-434.97234351
AAACGAA501.7327283E-434.92234450
Produced by FastQC (version 0.11.5)