FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964676
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43930.4553860570803047No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTACCAGTATCCCACAC40960.42459851805165666No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACG35770.3707980710622012No Hit
GGATTACCTGTGCGGGAAATAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC32840.34042517902383806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30590.3171012858203169No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27290.28289290912181914No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC23800.24671495921946848No Hit
CCCCTGTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCTCT22760.23593413747206318No Hit
GTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC21320.22100684582180963No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21040.21810431688981585No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT20310.21053700931711788No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20200.20939673009383464No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20170.20908574485112102No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19580.2029697017444199No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19370.20079280504542457No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT19060.1975792908707172No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCAAAGAGCTCAGTGTCAGGGA18520.19198155650187212No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17720.18368861669617573No Hit
CTATTATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA17580.18223735223017884No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGGATTAC17250.17881651456032907No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC16920.1753956768904793No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16880.17498102990019446No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCCCTCAGAGATCCCTGAGGGCCGGTCGCTATTATCATAGA16620.17228582446334315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16320.169175972036207No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA16150.1674137223274965No Hit
CCCCAGGAGAGACGGCCAGGATTACCTGTGCGGGAAATAACATTGGAAGT16000.1658587961139284No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15990.1657551343663572No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15860.16440753164793154No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15500.16067570873536816No Hit
GTATTATTGTGCGCGAGAGAGTGGGAAGCAGTGGCCACCTCAACAATATGACTCCTGGGGCCAGGGAACCCTGGT15350.15912078252180006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15340.15901712077422886No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15200.157565856308232No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15090.15642557708494875No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15040.1559072683470927No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14980.15528529786166548No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14730.15269375417238534No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG14080.14595574058025698No Hit
GTTTTACTTCCAATGTTATTTCCCGCACAGGTAATCCTGGCCGTCTCTCCTGGGGCCACTGACACCGAGGGTGG13890.1439861673764041No Hit
ATGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGA13840.14346785863854808No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13750.1425349029104072No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13700.1420165941725512No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13700.1420165941725512No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13540.14035800621141192No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATACTGGTA13270.13755913902698938No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA13140.1362115363085637No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA12880.13351633087171236No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGG12830.13299802213385636No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTA12790.13258337514357152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12730.1319614046581443No Hit
CTATTACTGTCAGGTGTGGGATACTGGTACTGATCATGTGTTATTCGGCG12680.13144309592028827No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12540.12999183145429138No Hit
TGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC12210.12657099378444162No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12110.12553437630872957No Hit
GTGCTGGTCGTCTATGATAATAGCGACCGGCCCTCAGGGATCTCTGAGGG12070.12511972931844473No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC12050.12491240582330233No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACACATGATCAGTAC11950.12387578834759028No Hit
ATCTCTGGGGGCGCCGTCAGTAGTGGTGGTTCCTACTGGAGCTGGATCCG11950.12387578834759028No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11910.12346114135730546No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11780.12211353863887979No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11750.12180255339616618No Hit
GTCCTGGACCTCCTGTGCAAGATCATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11510.11931467145445726No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC11270.11682678951274832No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT11230.1164121425224635No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC11180.11589383378460746No Hit
GATCATGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11090.11496087805646661No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11030.1143389075710394No Hit
GCCCTCAGGGATCTCTGAGGGATTCTCTGGCTCCAACTCTGGGAACACGG11030.1143389075710394No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAG10990.11392426058075458No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC10810.11205834912447288No Hit
ATACTGGTACTGATCATGTGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT10770.11164370213418806No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10720.11112539339633203No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10570.10957046718276396No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10500.1088448349497655No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAGAGACGG10450.1083265262119095No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10380.10760089397891105No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATAAT10290.1066679382507702No Hit
CCCTCGGTGTCAGTGGCCCCAGGAGAGACGGCCAGGATTACCTGTGCGGGAAATAACATTGGAAGTAAAACTGTG10280.10656427650319901No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10260.10635695300805659No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10220.10594230601777177No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10210.10583864427020057No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10170.10542399727991575No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10100.10469836504691732No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10060.10428371805663249No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9990.10355808582363404No Hit
CACATGATCAGTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGT9780.10138118912463873No Hit
GAATAACACATGATCAGTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTG9730.1008628803867827No Hit
GCTTGGTCCCTCCGCCGAATAACACATGATCAGTACCAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC9700.10055189514406909No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9650.10003358640621307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)