FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364201
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76040.5573958676177484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71530.5243362231811881No Hit
GGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATC63000.4618087803776716No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56430.4136487218525716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49970.36629499611860716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46350.3397593169921441No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43080.3157892422011126No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42870.3142498795998537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41980.30772591428975643No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAA41710.3057467338024235No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC41400.30347434139104135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40550.2972435880049934No Hit
GTTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG38210.2800906904481084No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTC37570.27539929966331944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36880.27034139397346874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36600.2682889105051235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36450.26718936578993857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36180.2652101853026057No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG36030.2641106405874208No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG35870.2629377928912235No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC34650.2539948292077194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34240.2509894069862139No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT33240.24365910888498105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33190.24329259397991937No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32820.24058038368246323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32740.23999395983436456No Hit
GCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTCCCGATCACCTTCGGCCAAGGGACACGA30510.22364739506861528No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGAAA29440.21580397610029609No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29000.21257864493575362No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26440.19381308179659743No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGA26170.19183390130926456No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC26160.1917605983282522No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA25880.189708114859907No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25810.18919499399282072No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA25550.18728911648650015No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24680.18091175713842755No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24640.18061854521437823No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24350.1784927587650207No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23590.1729217322080837No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23310.1708692487397385No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGG23160.16976970402455355No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC22960.16830364440430698No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22510.1650050102587522No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC22460.16463849535369054No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAAACTGTCAGCCTGTTGACAAAAGTAAGTTGCAAAGTCTTCAGG22050.16163307313218508No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21800.15980049860687684No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21490.15752810619549465No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21460.15730819725245768No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21000.15393626012589054No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTCCCGATCACCTT20950.1535697452208289No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20810.1525435034866563No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20630.15122404982843438No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20190.14799871866389191No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20180.1479254156828796No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGTTA19640.1439670547082138No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19610.14374714576517683No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19600.1436738427841645No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAG19600.1436738427841645No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19590.14360053980315218No Hit
GGCTGACAGTTTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT19470.14272090403100424No Hit
CTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACTCTCA19200.14074172354367134No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19100.14000869373354805No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18890.13846933113228915No Hit
CCCCTAAACTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCA18800.1378096043031782No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18760.13751639237912888No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT18700.1370765744930549No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18590.1362702417019193No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18530.1358304238158453No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18450.1352439999677467No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18440.13517069698673437No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18320.1342910612145864No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18120.13282500159433983No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC18110.1327516986133275No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17840.13077251812599464No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17800.1304793062019453No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCAGAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG17700.129746276391822No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTG17660.12945306446777272No Hit
AGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTG17410.1276204899424645No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17290.12674085417031655No Hit
CTGTCAGCCTGTTGACAAAAGTAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC17200.1260811273412056No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG17170.12586121839816858No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGACCTCATGTTCAGGGAGA17050.12498158262602066No Hit
GTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT17010.12468837070197135No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17010.12468837070197135No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16800.12314900810071244No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16730.12263588723362613No Hit
GGCCGAAGGTGATCGGGAAACTGTCAGCCTGTTGACAAAAGTAAGTTGCA16670.12219606934755216No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16380.12007028289819462No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16360.11992367693616995No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT15860.11625852788555353No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15680.11493907422733161No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15650.11471916528429463No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15520.11376622653113434No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15220.11156713710076446No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14980.10980786555646858No Hit
GTCTCTGGTGGCTCCATCAGTAGTCACTACTGGAGCTGGGTCCGGCAGCT14970.10973456257545626No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14910.10929474468938229No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14880.1090748357463453No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14790.10841510891723433No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14790.10841510891723433No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14540.10658253439192612No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGAAACTGTCAGCCTGTT14350.10518977775269187No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT13920.10203774956916173No Hit
ATGCAGCAGAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGTTAATACC13910.1019644465881494No Hit
TCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT13740.10071829591093981No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCAT13730.10064499292992748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC252.5143096E-455.43372343
GACGATA200.00731351751.8317541
TCGTATG1050.039.4292140
GTATCAA27050.038.4960751
CATTGAT6950.038.18021470
CGTATGC1151.8189894E-1236.0568741
TATCAAC30800.033.8028532
AATCTCG1056.85759E-1032.66462736
TGTTAGA19250.032.3936870
ATCAACG32950.031.5960483
GGTCTCG652.548743E-531.41463520
TATCTGG850.004235671431.21794170
TCAACGC33500.031.1788654
CAACGCA33700.030.9938285
GTGGTAT2200.030.9363881
AACGCAG33700.030.892876
GTATAGT1001.657645E-830.6270261
GTAGTTT1104.1000705E-430.15369270
AATGGTA6700.029.70363670
ACGCAGA35650.029.2009377
Produced by FastQC (version 0.11.5)