FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364201
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75170.5510185082696758No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61550.4511798481308839No Hit
GGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTGCTGCATC59410.43549301019424563No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52910.3878460725362318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51850.380075956548925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43440.31842814951755644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43340.31769511970743314No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42030.30809242919481805No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC39300.2880807153784523No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37930.2780382069797633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37560.2753259966823071No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37220.2728336953278879No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAA36920.27063460589751803No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG36910.2705613029165057No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTC36660.2687287283911975No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC36040.2641839435684331No Hit
GTTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG35470.26000567365073035No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34080.24981655929001667No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG33860.2482038937077454No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33530.24578489533433856No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33100.24263286715080842No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33050.24226635224574677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32210.23610890184071115No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31990.2344962362584399No Hit
GCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTCCCGATCACCTTCGGCCAAGGGACACGA30910.22657951430910842No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30570.22408721295468925No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA30330.22232794141039333No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30120.22078857880913444No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT28820.21125919127753168No Hit
CTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGA27020.1980646546953125No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGAAA26730.19593886824595497No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26580.19483932353077002No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26090.1912474774611659No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC26060.1910275685181289No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25540.18721581350548783No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAACT24800.1817913929105755No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24150.17702669914477412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24020.17607376039161385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23510.17233530835998506No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA22880.16771722055620836No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22850.16749731161317138No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22790.16705749372709738No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGAAACTGTCAGCCTGTTGACAAAAGTAAGTTGCAAAGTCTTCAGG22570.16544482814482617No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC22540.16522491920178917No Hit
GGCTGACAGTTTCCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT22320.16361225361951795No Hit
ATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGG21980.16111995226509876No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21640.15862765091067957No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21450.15723489427144532No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21420.15701498532840835No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT21310.15620865253727273No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21140.15496250186006313No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20930.15342313925880424No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC20660.15144395877147135No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20400.1495380812651508No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19770.1449199934613741No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19450.14257429806897956No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19430.1424276921069549No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTACTTTTGTCAACAGGCTGACAGTTTCCCGATCACCTT19150.14037520863860972No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19050.13964217882848642No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAACAG18970.1390557549803878No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18950.13890914901836313No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGTTA18940.1388358460373508No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18800.1378096043031782No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18720.13722318045507956No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18600.13634354468293164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18530.1358304238158453No Hit
CTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACTCTCA18280.1339978492905371No Hit
GGCCGAAGGTGATCGGGAAACTGTCAGCCTGTTGACAAAAGTAAGTTGCA17580.1288666406196741No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17470.12806030782853847No Hit
CTGTCAGCCTGTTGACAAAAGTAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTC17370.12732727801841517No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17320.12696076311335353No Hit
CCCCTAAACTCCTGATCTCTGCTGCATCCAGTTTGCAAAGTGGGGTCCCA17270.12659424820829188No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17210.12615443032221793No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17200.1260811273412056No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17040.12490827964500834No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCAGAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG16950.12424855281589736No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16890.12380873492982339No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16740.12270919021463846No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16410.12029019184123162No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG16410.12029019184123162No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC16330.11970376799313298No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTG16150.11838431433491105No Hit
AGTCAGGGTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTCTG16090.11794449644883709No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16030.11750467856276312No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGACCTCATGTTCAGGGAGA15970.11706486067668913No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15950.11691825471466448No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15940.11684495173365216No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15930.11677164875263982No Hit
GTATTAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT15790.11574540701846721No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15560.11405943845518365No Hit
GTCTCTGGTGGCTCCATCAGTAGTCACTACTGGAGCTGGGTCCGGCAGCT15440.11317980268303572No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15440.11317980268303572No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15300.11215356094886311No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15070.11046759238557956No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14940.10951465363241927No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14860.10892822978432065No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTG14230.10431014198054392No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14090.10328390024637132No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13870.10167123466410008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT959.113137E-1056.4756870
GTGGTAT3800.039.3584371
GGTACGT1001.0186341E-1038.99507568
CATTGAT5800.037.00130570
GTATCAA30650.036.708471
AATGGTA7300.033.9917870
TACTCAT800.00298403833.53243670
GTACGTA1208.516508E-932.79252669
TATCAAC34450.032.251662
TGGTACG1158.021743E-1032.18412867
ATCAACG35100.031.5448743
TCAACGC35750.030.9701964
TCGTAGG450.004511832730.81957242
CAACGCA36250.030.6367115
AACGCAG36150.030.627516
TGGATTT8900.030.14151470
ACCTTAT1401.2732926E-1129.7589743
CGCAGAG37750.029.4204398
TATGTAT1451.6370905E-1129.28039256
ACGCAGA38150.029.0218757
Produced by FastQC (version 0.11.5)