FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314679
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGCTCACCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40470.30783179772400715No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC37460.28493647498743035No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT35830.2725380111799154No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35220.2678980952764895No Hit
CTACTATACTGCTGACAGTAATATGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGCTGAGTGAGAAGTACGTC34100.25937890542101916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30630.23298462970808842No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27920.21237123282565554No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTAGCAGCAGTTACTTAGCCTGGTACC27230.20712280336112465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24880.1892477175036644No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24710.1879546261863162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24250.18445567320996226No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23960.18224981155095654No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG23590.17943543633084577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21630.16452685408377254No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT21020.1598869381803467No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19300.14680389661658855No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT19040.14482622754299718No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTTAGCAG19020.14467409915272092No Hit
ATATTACTGTCAGCAGTATAGTAGCTCACCTCACACTTTCGGCGGAGGGA18900.14376132881106338No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA18880.14360920042078712No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA18750.14262036588399146No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18730.14246823749371518No Hit
CAGTAATATGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGCTGAGTGAGAAGTACGTCCCAGACCCACTGCCA18620.14163153134719578No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18570.14125121037150515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18410.14003418324929506No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18240.1387410919319469No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACTGCTGCTAACACTCTGA18190.13836077095625623No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17120.13022190207647646No Hit
GAATATCTCTGATCCCACCTCACCATTGAGAACCAGATTTGTGTACCATT16940.12885274656399015No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16920.1287006181737139No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16680.12687507749039878No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16630.12649475651470815No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16510.1255819861730506No Hit
CTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCT16490.12542985778277435No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTG16460.12520166519735995No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16320.12413676646542617No Hit
CTACAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG16250.12360431709945927No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC16240.12352825290432112No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGAGAATATCTCTGATC16200.12322399612376862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16050.1220830331966967No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC15750.11980110734255281No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC15370.11691066792730394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15240.11592183339050824No Hit
GTGTAGCAGGTCTCTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAG15050.11447661368288381No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT15000.11409629270719317No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAACAGGGAGAATATCTCTGATCCCAC14670.11158617426763491No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT14550.11067340392597737No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT14480.11014095456001047No Hit
GAGATATTCTCCCTGTTGTTCAGAGGAACAATAATTCGGATGTTTCTCTC14430.10976063358431982No Hit
GTAATATGCTGCAAAATCTTCAGGCTCCAGCCTGCTGATGCTGAGTGAGAAGTACGTCCCAGACCCACTGCCA14020.10664200158365654No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGA13810.10504465348575584No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13670.10397975475382204No Hit
GATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTG13550.10306698441216448No Hit
GCATATTACTGTCAGCAGTATAGTAGCTCACCTCACACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13450.1023063424607832No Hit
CTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGAT13360.10162176470454004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)