FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280819
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT132621.0354312357952216No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTC99960.7804381415328786No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75040.5858751314588556No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT73820.576349976069999No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67670.5283338239048608No Hit
CTTTCATACAGCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAA66560.5196674940018847No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTGTATGAAAGACT55790.4355806714297648No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48800.38100621555426645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48510.3787420392733087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48060.3752286622856157No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44300.34587244567733616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43100.3365034403768214No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42930.3351761646259151No Hit
CTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTT41980.3277590354296743No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGA40140.3133932273022183No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39390.30753759898939664No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37130.2898926390067605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36010.2811482340596134No Hit
GTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA35450.27677603158603986No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC34900.27248190415663726No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATA33510.26162947301687434No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33440.2610829477076777No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33100.25842839620586516No Hit
GTATGAAAGACTCTGACTGGACTTGCAGCTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA32700.2553053944390269No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31680.24734173993358938No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31460.24562408896182833No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30920.24140803657659668No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30920.24140803657659668No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA30830.2407053611790581No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29710.231960956231911No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC29290.22868180437673083No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATACAGCTCCAA29170.22774490384667936No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27430.21415984616093295No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26570.2074453923622307No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCT25990.20291703980031528No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25520.19924751272428032No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25250.19713948653166452No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA25000.1951876104273906No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTT24600.19206460866055236No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24110.1882389314961755No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23740.1853501548618501No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGT23650.1846474794643115No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23610.1843351792876277No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT23390.18261752831586664No Hit
ATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA22580.1762934497380192No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22210.1734046731036938No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22140.17285814779449712No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA21590.16856402036509452No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATACAGCTCCAACAGCAAGACCTA21530.1680955701000688No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21310.16637791912830774No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21190.16544101859825627No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTG21090.16466026815654672No Hit
GCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGG20850.16278646709644376No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20490.15997576550628934No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20460.15974154037377647No Hit
GAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAAT20000.15615008834191246No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19690.15372976197261284No Hit
GACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGC19630.1532613117075871No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19630.1532613117075871No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19520.1524024862217066No Hit
GCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGT19460.15193403595668084No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19350.15107521047080033No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19150.1495137095873812No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18950.14795220870396208No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18830.1470153081739106No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18770.14654685790888486No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18750.14639070782054295No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTG18490.14436075667209808No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18470.14420460658375617No Hit
TCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACC18450.14404845649541426No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18430.14389230640707235No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT18400.14365808127455948No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18250.14248695561199515No Hit
CAGCAAGACCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTAC18130.14155005508194365No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17870.13952010393349878No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17730.1384270533151054No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17600.137412077740883No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17550.13702170252002818No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17530.13686555243168627No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17160.13397677579736092No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16630.12983879845630023No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA16520.12897997297041972No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCG16370.12780884730785536No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16160.1261692713802653No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16050.12531044589438475No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16040.1252323708502138No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15950.1245296954526752No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15930.12437354536433329No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15870.12390509509930756No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15840.12367086996679469No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15400.12023556802327261No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCGGATTCCCGGGT15060.1175810165214601No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15060.1175810165214601No Hit
GTCTGGGACAGATTTCACTCTCTCCATCAGCAGCCTGCAGGCTGAAGATG15020.11726871634477626No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14670.11453608979879279No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14570.11375533935708323No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14440.11274036378286081No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14340.11195961334115126No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14240.11117886289944169No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCC14100.1100858122810483No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14050.10969543706019352No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13870.10829008626511631No Hit
CATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCA13850.1081339361767744No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA13600.10618206007250049No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC13580.10602590998415858No Hit
CTCTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCA13480.10524515954244901No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13420.10477670927742327No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13410.10469863423325232No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCAGC13380.10446440910073945No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13320.1039959588357137No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13260.10352750857068797No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13180.10290290821732033No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13160.1027467581289784No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGGGGAGGAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC13140.1025906080406365No Hit
AAGTAGGTCTTGCTGTTGGAGCTGTATGAAAGACTCTGACTGGACTTGCA13140.1025906080406365No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13100.10227830786395267No Hit
ATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCAGCC13060.10196600768726885No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13000.1014975574222431No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA12900.10071680698053355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT209.0470094E-568.1598741
GGTATCA12150.048.5253451
TCGTATG1250.046.95010440
GTGGTAT2750.042.1351971
GCGAATC1550.042.01001433
GACTAAG1800.041.68744326
TGGTATC2950.040.4227752
GGCGAAT1800.039.9471332
GAGACTA1800.039.76303524
CGAGACT1800.039.7537223
TGCCGTC1400.039.70833645
TAAGGCG1900.039.5397329
ACTAAGG1900.039.51035727
ATCTCGT1400.039.30150237
AAGGGTA350.00137367839.27673736
GTATCAA30600.039.0916941
ACGAGAC1900.037.66141522
CGTATGC1500.036.8702841
GCCGTCT1450.035.99017746
TTATACA2000.035.774162
Produced by FastQC (version 0.11.5)