FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501264
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT153081.0196740879685384No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCT134130.8934471218919524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC112700.7507007428406997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT105430.7022748830318984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC87940.5857730552387854No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATAGTGAGAGTGAAGTCTGTC73440.4891877777659359No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC72340.48186061878523695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA71280.4747999019492907No Hit
GTTTAGGAGGCTGTCCGGGTTTCTTCTGGAACCAAGCCAAGTAGTTCTCA69190.46087829988596274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65900.4389634334800541No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG65090.43356798004881225No Hit
CTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCA64780.4315030534269789No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG64190.4275730317918767No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT61250.4079895341525541No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG57310.3817449828944143No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56970.37948022466401643No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT56600.3770156348250541No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT55370.3688225388739089No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT52700.35103752571166696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC51670.34417664048428526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG49590.33032164895714544No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG45890.3056757505675218No Hit
GGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC44300.2950846753136024No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43130.28729124257958627No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC41350.2754345671380916No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40760.2715045455029895No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC40140.2673746922593228No Hit
CACTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC39500.26311161794327975No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38710.25784938558441417No Hit
ATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGA37490.24972290016945722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA36850.24545982585341417No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT36630.2439943940572744No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG36620.24392778352108624No Hit
GTTTATTACTGTCAACAATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAA35920.23926504598791418No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA33940.2260761598226561No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA33330.22201291711517762No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33240.22141342228948405No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC33220.22128020121710773No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT33220.22128020121710773No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC33120.22061409585522598No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA32740.21808289548007545No Hit
CTACTTGGCTTGGTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGC32720.2179496744076991No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC31960.212887273657398No Hit
GGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGCGGACATCGTGATGA31330.2086908098775432No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC31220.20795809397947332No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT31020.20662588325570985No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT30160.20089737714352707No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC30100.20049771392639804No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT29890.1990988926664464No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAACAGCC28780.1917051231495593No Hit
GACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTAAG28780.1917051231495593No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC28770.19163851261337114No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTG28690.19110562832386574No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28680.1910390177876776No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27850.1855103432840593No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG27590.18377846934316683No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA27560.18357863773460228No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG27460.1829125323727206No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27310.181913374329898No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27190.18111404789563995No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC27100.1805145530699464No Hit
GTCTGGGACAGACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGATG26860.17891590020143025No Hit
CTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAA26490.17645131036246792No Hit
AAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT26200.1745196048130109No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25750.17152213068454314No Hit
GTATAGAAAAGACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCC25450.169523814598898No Hit
ATATAAATGAGAACTACTTGGCTTGGTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCAT25190.1677919406580055No Hit
GGCCAAAAGTGTGAGGCTTAGTATAATATTGTTGACAGTAATAAACTGCC25040.16679278261518296No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG24870.165660403499984No Hit
GTCAGGGACCCCGGCCTCCCGGGCAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCGGGTTTCTTCTGGAA24450.1628627609800808No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC24430.16272953990770445No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG24370.1623298766905754No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT24370.1623298766905754No Hit
GCCCGGGAGGCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAACAGC24320.16199682400963455No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT23750.15820002344690876No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23220.15466966502893562No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22510.14994031695957538No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC22510.14994031695957538No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21770.14501113728165066No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21730.14474469513689797No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT21490.14314604226838185No Hit
CCCAGAGACACAGCCAGGGATTCTGGAGACTGCGTCATCACGATGTCCGC21390.14247993690650013No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT21130.14074806296560766No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC20860.138949578488527No Hit
GGTCTGGGACAGACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGAT20800.13854991527139796No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG20670.13768397830095175No Hit
GCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGG20530.1367514307943173No Hit
GTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGT20480.13641837811337645No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC20420.13601871489624742No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20370.13568566221530656No Hit
GCTTAGTCCCCTGGCCAAAAGTGTGAGGCTTAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC20110.1339537882744141No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19830.13208869326114528No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT19680.1310895352183227No Hit
GCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTT19490.12982393503074743No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGCCTCCCGGGC19020.12669323982990335No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18920.1260271344680216No Hit
GACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT18800.12522780803376354No Hit
GTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCTGA18640.1241620394547528No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT18600.1238955973100001No Hit
GGCAGGGGCAGCAGGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18340.12216372336910764No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18170.12103134425390871No Hit
CTCCAGAATCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTCTTTTCTATA18160.12096473371772053No Hit
ATTCTGGAGACTGCGTCATCACGATGTCCGCGTAGGCACCAGAGATCCAG18000.11989896513870978No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT17940.11949930192158074No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTAGTCC17930.11943269138539259No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG17820.11869997548732268No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG17370.11570250135885495No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA17130.1141038484903388No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA17040.11350435366464526No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG17010.11330452205608076No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16670.11103976382568288No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG16650.11090654275330654No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT16520.11004060578286032No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG16470.10970755310191944No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG16290.10850856345053235No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAG16270.10837534237815599No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAA16180.10777584755246244No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC16130.1074427948715216No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA15780.10511142610493557No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC15720.10471176288780655No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG15660.1043120996706775No Hit
GCCACAGTTCGTCTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTGTGAGG15480.10311311001929041No Hit
GAACTACTTGGCTTGGTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGC15200.10124801500602158No Hit
GAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15030.10011563589082267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)