FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501264
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT147740.9841040616440546No Hit
CTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCT121810.8113829413081244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99780.6646399300855812No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC94770.631268051455307No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC86970.5793118332285327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC75930.5057738012767907No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATAGTGAGAGTGAAGTCTGTC71370.4753993967749843No Hit
GTTTAGGAGGCTGTCCGGGTTTCTTCTGGAACCAAGCCAAGTAGTTCTCA66750.4446253290560488No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA65490.4362324014963391No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC65410.4356995172068337No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT63180.42084536763687136No Hit
CTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCA63090.42024587281117776No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT60490.402927133402253No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG57470.3828107514734251No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG55370.3688225388739089No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT55040.36662439117969925No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC53300.35503415788295734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50790.33831491329972607No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT49970.33285284933229603No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48920.3258587430325379No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT47850.31873141566040347No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC46500.30973899327500026No Hit
GGACTAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATC43660.2908216009975594No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG42610.28382749469780133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42390.28236206290166155No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42380.2822954523654734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40900.2724370930096239No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG40160.26750791333169915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39950.26610909207174754No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC38830.2586487120186723No Hit
GTTTATTACTGTCAACAATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAA37620.25058883713990343No Hit
CACTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC37150.24745814193905935No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA36230.24132997260974753No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGCCTCCCGGGC36060.24019759349454858No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA35420.23593451917850558No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG34670.2309387289643927No Hit
GGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGCGGACATCGTGATGA34510.2298729603853819No Hit
ATACTAAGCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGA34000.22647582303978514No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33400.22247919086849482No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT32350.21548508456873677No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA32300.21515203188779589No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT32160.21421948438116148No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC31830.2120213366869518No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC31780.21168828400601092No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC31690.2110887891803174No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA31290.2084243677327905No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC31240.20809131505184963No Hit
GACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTAAG30800.20516045145957004No Hit
GTCTGGGACAGACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGATG30550.20349518805486574No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30370.20229619840347865No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC29710.1978999030150593No Hit
GTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGT28690.19110562832386574No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTG27620.18397830095173134No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27530.1833788061260378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26590.1771174157243496No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26480.17638469982627972No Hit
CTATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAA26420.17598503660915069No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAACAGCC26190.17445299427682273No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT25990.1731207835530593No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25970.17298756248068295No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT25820.17198840443786034No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25730.1713889096121668No Hit
CTACTTGGCTTGGTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGC25610.17058958317790876No Hit
GGCCAAAAGTGTGAGGCTTAGTATAATATTGTTGACAGTAATAAACTGCC25420.1693239829903335No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC25000.16652634047043025No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24030.16006511846017754No Hit
AAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT23950.1595322341706722No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT23880.159065960417355No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA23650.157533918085027No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23410.15593526521651088No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23350.15553560199938185No Hit
GCCCGGGAGGCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAACAGC23260.1549361071736883No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA23060.15360389644992486No Hit
GTCAGGGACCCCGGCCTCCCGGGCAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCGGGTTTCTTCTGGAA22610.15060642232145713No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21690.14447825299214528No Hit
ATATAAATGAGAACTACTTGGCTTGGTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCAT21460.14294621065981733No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG21370.14234671583412378No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG21130.14074806296560766No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21100.14054823135704314No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21070.14034839974847862No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG20920.13934924170565605No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG20920.13934924170565605No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20760.1382834731266453No Hit
GGTCTGGGACAGACTTCACTCTCACTATCAACAGCCTGCAGGCTGAAGAT20390.1358188832876829No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTAAGCCTCACACTTTTGGCCAGGGGACTAAG20350.13555244114293022No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20280.13508616738961302No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG20220.13468650417248398No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20130.13408700934679044No Hit
GCCTCACACTTTTGGCCAGGGGACTAAGCTGGAGATCAGACGAACTGTGG20080.13375395666584958No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT19860.1322885248697098No Hit
GCTTAGTCCCCTGGCCAAAAGTGTGAGGCTTAGTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGC19550.13022359824787644No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG19490.12982393503074743No Hit
GGCAGGGGCAGCAGGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19360.1289579980603012No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA18780.1250945869613872No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18550.12356254462905926No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC18370.12236355497767216No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18280.12176406015197859No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18240.12149761800722592No Hit
GCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTT18090.12049845996440334No Hit
GTATAGAAAAGACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCC17960.11963252299395709No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAGG17850.1188998070958872No Hit
GACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT17670.1177008174445001No Hit
TCTTCAGCCTGCAGGCTGTTGATAGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17420.1160355540397958No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG17350.1155692802864786No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17240.11483656438840871No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17230.11476995385222052No Hit
CTCCAGAATCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTCTTTTCTATA16960.11297146937513988No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16890.11250519562182268No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC16760.11163925865137643No Hit
ATTCTGGAGACTGCGTCATCACGATGTCCGCGTAGGCACCAGAGATCCAG16460.10964094256573129No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT16360.10897483720384955No Hit
GTTCCAGAAGAAACCCGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTGCCCGGGAGGCCGGGGTCCCTGA16270.10837534237815599No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG16200.1079090686248388No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15550.10357938377260761No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA15400.10258022572978502No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACTATCAA15350.10224717304884418No Hit
CCCAGAGACACAGCCAGGGATTCTGGAGACTGCGTCATCACGATGTCCGC15340.102180562512656No Hit
GCCACAGTTCGTCTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTGTGAGG15340.102180562512656No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTAGTCCCCTGGCCAAAAGTGTGAGGCTTAGT15090.10051529910795169No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG15090.10051529910795169No Hit
GGATCTCTGGTGCCTACGCGGACATCGTGATGACGCAGTCTCCAGAATCCCTGGCTGTGTCTCTGGGCGAGAGGG15060.10031546749938718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTT200.00773580151.10125436
TCCGAAC200.00815898750.41735522
CCGTAGT306.2818057E-446.04480442
GGTATCA8750.045.6629681
AATGGTA4900.044.72934370
TACGTAT457.9961865E-539.82787355
AGAAAAG25900.039.40262270
GTATCAA20350.035.473111
GGTCTGG46050.035.24985570
TAGAAAA26100.032.08553369
GTGGTAT2350.031.432581
AGTATAG22250.031.28202865
TGGTATC2500.030.8783322
ATTCACT3050.030.8123052
TGTTAGA34300.030.75142370
GAAAGTA22050.030.59061662
AAGTATA22500.030.28248464
AAAGTAT22450.030.057463
CGCACGG500.00520111529.93656765
TCCTAAC450.005252237429.87695322
Produced by FastQC (version 0.11.5)