FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170784
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCTGGGGTCCCT71410.60993317298494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70050.5983170251728757No Hit
CTATTGTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGACCAAACTGG66700.5697037199005112No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66250.5658601415803427No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCAAGGAGTTCTTATTGTTGGAGCTGCAAAAAACACT57030.48710949244267093No Hit
ATTGTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT52740.45046737912373247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49930.42646636783557No Hit
GTACTACAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT44850.38307663924344715No Hit
CTACAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC44280.3782081067045672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44180.37735397818897426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43990.37573113400934754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43480.37137507857982344No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41620.35548828818979417No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38860.3319143411594282No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38730.33080397408915735No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36060.307998742722825No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34960.2986033290513024No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34090.29117241096564356No Hit
GTTTATTACTGTCAGCACTATTGTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAA33170.28331442862218825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32800.2801541531144942No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA31140.2659756197556509No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31010.2648652526853801No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30420.25982589444338156No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA30180.2577759860059584No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30090.2570072703419247No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTG29680.2535053434279936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29630.25307827917019704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28380.24240167272528493No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA27980.23898515866291303No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27940.23864350725667588No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTGCAGCTCCAA27580.2355686446005412No Hit
TTGTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT26740.22839396506956025No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC26180.2236108453822396No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25890.22113387268701995No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT25660.21916937710115617No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC25160.2148987345231913No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAAGTG24900.21267800038264956No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24850.2122509361248531No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24500.20926148632027772No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23580.20140350397682238No Hit
GTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT23360.19952442124251785No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTGTAGTACT22930.19585166862546807No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22840.1950829529614344No Hit
GGCCAAAAGTGTACATGGGAGGAGTACTACAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGG21850.186627080657064No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCA21830.18645625495394538No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC21820.18637084210238608No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21640.18483341077431875No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20480.1749255199934403No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20320.17355891436849152No Hit
GTCCTGGTTTCTGCTGGTACCAAGCCAAGGAGTTCTTATTGTTGGAGCTG20210.17261937300133928No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTGCAGCTCCAACAATAAGAACTC19990.17074029026703474No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19970.17056946456391614No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19630.16766542761090006No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19460.166213409134392No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19120.1633093721813759No Hit
GAGTACTACAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18780.16040533522835979No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18570.15861166534561455No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18440.1575012982753437No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18400.15715964686910652No Hit
CAATAAGAACTCCTTGGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTAC18390.15707423401754722No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18320.15647634405663213No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18260.15596386694727635No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18260.15596386694727635No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17770.1517786372208708No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17650.15075368300215924No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17540.14981414163500698No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17510.14955790308032907No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTGTAGTACTCCTCCCATGTA17390.14853294886161753No Hit
GTATATTACTGTGCGAGAGATCAGCTGAGGAGTGGGACCTACGAGGGGTGGGGCCAGGGAACCCTGGTCACCGT17290.14767882034602456No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17200.14691010468199087No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17150.14648304042419438No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16960.14486019624456775No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16860.14400606772897476No Hit
CTCCTTGGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAAT16810.14357900347117827No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16730.1428957006587039No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16720.1428102878071446No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16490.14084579222128077No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGAGCTGGATCCG16470.1406749665181622No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16380.13990625085412853No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16310.13930836089321344No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16170.1381125809713833No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16040.13700221390111242No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15890.13572102112772297No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15740.1344398283543335No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15670.13384193839341843No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15630.13350028698718125No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15210.12991294722169078No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15160.12948588296389427No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG15010.12820469019050482No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14720.12572771749528522No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA14710.12564230464372592No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14620.12487358897969225No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14570.12444652472189575No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCTGGGGTCCCTGA14310.12222579058135404No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14270.12188413917511684No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14150.12085918495640527No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13980.11940716647989723No Hit
GTCAGGGACCCCAGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13950.11915092792521934No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA13930.11898010222210074No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13860.11838221226118566No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG13750.1174426708940334No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT13730.1172718451909148No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13540.11564900101128817No Hit
GTGTACATGGGAGGAGTACTACAATAGTGCTGACAGTAATAAACTGCCAC13340.11394074398010223No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCTGGGGTCCCTGACCGATT13340.11394074398010223No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13270.11334285401918714No Hit
GCCAAGGAGTTCTTATTGTTGGAGCTGCAAAAAACACTCTGGCTGGACTT13140.11223248694891629No Hit
CATGTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGG13070.1116345969880012No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG13030.111292945581764No Hit
CATTTACTGGGCATCTACCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA13030.111292945581764No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTGTAGTACTCCTCCCATGTACACTTTTGGCCAGGGGAC12990.11095129417552682No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCAGATTCCCGGGT12990.11095129417552682No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT12900.11018257851149314No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12880.11001175280837457No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12830.10958468855057808No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12570.10736395441003635No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12380.10574111023040972No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12350.10548487167573181No Hit
GTACACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTG12300.10505780741793533No Hit
ACCCGGGAATCTGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC12220.10437450460546095No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12090.10326413753519009No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12050.10292248612895291No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12050.10292248612895291No Hit
GGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11940.10198294476180063No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTGCAGCTCCAACAATAA11830.10104340339464837No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11760.1004455134337333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)