FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096042
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39710.3623036343497786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37890.34569843126449534No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28960.26422345129110014No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27970.2551909507117428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25910.23639605051631232No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24700.22535632758598667No Hit
GTGTTAACCCCAGCTACACAGCCTGGTACCAGCAGAGACCTGGCCAGGCT24240.22115940812487112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24090.21979084743102908No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23640.21568516534950302No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23030.2101196851945455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22200.2025469826886196No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT21990.20063099771724077No Hit
GTATATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGG21770.1986237753662725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21420.1954304670806411No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21080.19232839617459915No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20720.1890438505093783No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGGCTGAAGATT20270.1849381684278522No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20240.18466445628908382No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19780.18046753682796826No Hit
GTATTTCTGTCAGCAATATGATAACTCACGGTACACTTTTGGCCAGGGGA19690.17964640041166308No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19570.17855155185658944No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18940.17280359694245293No Hit
GGTATATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGG18680.17043142507312675No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18350.1674205915466743No Hit
GTATATACCATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGT17230.15720200503265386No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16760.15291384819194884No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16190.14771331755534914No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG16170.1475308427961702No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16160.14743960541658074No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16140.1472571306574018No Hit
GCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTATATACAGGAGGGCCACTGGCATC16090.14680094375945446No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15910.14515867092684404No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15070.13749473104132873No Hit
ATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG14890.13585245820871827No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGGCTGAAGATTTTGCAGTGTATTTCTGTCAGCAATATGATAACTCA14720.13430142275569734No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14490.13220296302513956No Hit
GGCCAAAAGTGTACCGTGAGTTATCATATTGCTGACAGAAATACACTGCA14340.1308344023312975No Hit
GTGTATTTCTGTCAGCAATATGATAACTCACGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14240.12992202853540283No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14200.12955707901704497No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13840.12627253335182412No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13660.12463026051921368No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13450.12271427554783483No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13390.12216685127029803No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13270.12107200271522442No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGAC13180.1202508662989192No Hit
CAGAAATACACTGCAAAATCTTCAGCCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13120.11970344202138239No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13080.11933849250302452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12830.11705755801328781No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCACAGTGTTAACCC12800.1167838458745194No Hit
AAAGTGTACCGTGAGTTATCATATTGCTGACAGAAATACACTGCAAAATC12660.11550652256026685No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACTGAGTTCAGTTGAAGGGAGA12620.11514157304190899No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTCCT12570.11468538614396165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12440.11349930020929855No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12130.11067094144202502No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12050.10994104240530929No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGC11910.10866371909105672No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11890.10848124433187778No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11870.10829876957269885No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11660.10638278460132003No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11630.10610907246255162No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11600.1058353603237832No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11510.105014223907478No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA11230.10245957727897288No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCACA11140.10163844086266767No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11110.10136472872389928No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11100.1012734913443098No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11070.1009997792055414No Hit
GTCTATTACTGTGCGAGAGAACTCCGGGGACCTATTTCCGGCATTGGATCTCCACATCACTTTGACTCTTGGGGC11060.10090854182595194No Hit
CCTGTATATACCATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCT10980.10017864278923617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG200.007046998552.31989745
ATACCGC200.00809144450.522597
TCGTATG4050.049.42381340
CGTATGC4100.048.92628541
GGTATCA10400.046.9858251
TATGCCG4350.045.48768643
CCCACGA4350.044.15280519
ACGAGAC4400.043.6650222
ATGCCGT4600.043.1067444
CGAGACG4500.042.6985923
ATCTCCG4700.041.56468610
TCCGAGC4750.041.1290413
GTATCAA32300.040.3776171
ACGGAGC4900.039.98857527
TTATACA4900.039.8754462
GTATGCC5250.039.6157342
TATACAC5400.039.302523
GGAGCTA5000.039.25886529
CCGTCTT5000.039.24350747
AGACGGA5000.039.13686825
Produced by FastQC (version 0.11.5)