FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096042
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39550.3608438362763471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38680.3529061842520633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32930.3004446909881191No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29200.2664131484012474No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.25318372836077446No Hit
GTGTTAACCCCAGCTACACAGCCTGGTACCAGCAGAGACCTGGCCAGGCT24830.22654241352064977No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24190.22070322122692382No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23580.2151377410719662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21770.1986237753662725No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21580.19689026515407257No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21040.19196344665624127No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGGCTGAAGATT20560.18758405243594678No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20450.18658044126046264No Hit
GTATATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGG19510.17800412757905262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19440.17736546592192634No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19420.1771829911627474No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19340.17645309212603166No Hit
GTATTTCTGTCAGCAATATGATAACTCACGGTACACTTTTGGCCAGGGGA18660.17024895031394782No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18540.16915410175887421No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18290.1668731672691375No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18220.16623450561201122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18020.16440975802022184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17270.15756695455101175No Hit
GGTATATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGG17110.15610715647758022No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17030.1553772574408645No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16600.15145405011851737No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16100.14689218113904395No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16040.14634475686150714No Hit
GTATATACCATACATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGT15770.1438813476125915No Hit
GCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTATATACAGGAGGGCCACTGGCATC15570.14205660002080212No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15220.13886329173517073No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG15140.13813339269845498No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15060.13740349366173923No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14780.13484884703323413No Hit
GGCCAAAAGTGTACCGTGAGTTATCATATTGCTGACAGAAATACACTGCA14640.13357152371898157No Hit
GTGTATTTCTGTCAGCAATATGATAACTCACGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14490.13220296302513956No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14430.13165553874760275No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGGCTGAAGATTTTGCAGTGTATTTCTGTCAGCAATATGATAACTCA14040.12809728094361347No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14010.12782356880484508No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCACAGTGTTAACCC13500.1231704624457822No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGAC13500.1231704624457822No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13370.1219843765111191No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12910.11778745705000357No Hit
ATACAGGAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG12770.116510133735751No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12690.11578023469903526No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12590.11486786090314058No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTCCT12530.11432043662560375No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12430.11340806282970908No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG12190.11121836571956184No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12160.11094465358079343No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12010.10957609288695142No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACTGAGTTCAGTTGAAGGGAGA11900.10857248171146724No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGC11450.10446679962994118No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11420.10419308749117279No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11390.10391937535240438No Hit
CAGAAATACACTGCAAAATCTTCAGCCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11140.10163844086266767No Hit
AAAGTGTACCGTGAGTTATCATATTGCTGACAGAAATACACTGCAAAATC11020.10054359230759406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11000.10036111754841512No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC10990.10026988016882565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)