FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830117
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA149380.8162319676829405No Hit
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT128620.7027965971574495No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC113380.6195232326676382No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA110090.6015462399398508No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT101580.5550464806348447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC98200.536577716069519No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT74100.4048921462398306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC73590.40210543916044716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC69500.3797571412100975No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66860.365331833975642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA64220.3509065267411865No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT63910.3492126459674436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT61770.3375194044970895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC60310.3295417724659134No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG55420.3028221692930015No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG55100.30107364720397656No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG53020.28970825362531466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT50870.27796037083967856No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT49410.2699827388085024No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT48890.26714139041383694No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA48020.26238759598430045No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC47410.2590544757520967No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG47020.25692346445609765No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45110.24648697323723018No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC44750.24451988588707718No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAA43960.2402032219797969No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC43870.23971145014225867No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG43250.2363236885947729No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC43160.23583191675723464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG41240.22534078422308518No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC40840.22315513161180406No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTA40300.2202045005865745No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA38720.21157117277201404No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA37090.2026646383810434No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC36690.20047898576976225No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35980.19659945238473825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34490.188457896407716No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG34170.1867093743186911No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT33930.1853979827519224No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT33790.184633004337974No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32850.17949672070146336No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA32810.17927815544033524No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31690.17315832812874807No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA31180.1703716210493646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30950.16911487079787796No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA30200.1650167721517258No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA30070.16430643505305945No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA29690.16223006507234236No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC29160.15933407536239486No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28610.1563288030218833No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG28520.15583703118434505No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT28500.155727748553781No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT28120.1536513785730639No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT28070.15337817199665377No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG28000.15299568278967957No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT27610.15086467149368046No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC27430.14988112781860394No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA27230.14878830151296338No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC27070.14791404046845094No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG26860.14676657284752834No Hit
CTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATC26800.14643872495583615No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC26430.1444169962904011No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT26160.14294168077778635No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCA26160.14294168077778635No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC26100.14261383288609417No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC25650.14015497369840288No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA25490.13928071265389044No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTT25480.13922607133860843No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25470.13917143002332638No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT25440.1390075060774803No Hit
ACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATT25360.13857037555522406No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC25130.13731362530373742No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25050.13687649478148117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCC24930.13622079899809683No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG24850.1357836684758406No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24760.13529189663830235No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTA24700.1349640487466102No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG24570.13425371164794384No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT24070.1315216458838424No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23920.13070202615461196No Hit
AATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA23860.1303741782629198No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG23740.12971848247953546No Hit
ATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGA23610.1290081453808691No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC23460.12818852565163866No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC22650.12376257911379436No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22570.12332544859153813No Hit
GCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC22410.12245118754702568No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22160.12108515466497498No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT21620.11813452363974544No Hit
GAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGC21320.11649528418128459No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC21210.11589422971318226No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21150.1155663818214901No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC21110.11534781656036198No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG20930.11436427288528549No Hit
GCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTC20890.11414570762415736No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTAC20780.11354465315605505No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTA20780.11354465315605505No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC20710.11316216394908085No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20680.11299824000323476No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC20660.11288895737267071No Hit
GTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG20660.11288895737267071No Hit
CCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG20650.11283431605738868No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT20130.1099929676627232No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT20060.109610478455749No Hit
ACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC19600.1070969779527757No Hit
GCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTT19320.1055670211248789No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19130.10452883613452037No Hit
GGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATC19130.10452883613452037No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC18610.10168748773985489No Hit
GAATCGGTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTG18590.10157820510929083No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18520.10119571590231663No Hit
GTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCAGAGATCCAGAGCAACAGAGAAATGAAGACCTGGGT18410.1005946614342143No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA18350.10026681354252213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)