FastQCFastQC Report
Wed 8 May 2024
EGAF00004691554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830117
Total Bases134.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA149240.815466989268992No Hit
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCC142930.7809883193260322No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT136350.7450343338704575No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT111030.6066825235763615No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA110790.6053711320095928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC101950.5570682093002797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC82980.4534136342102718No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC79360.4336334780781775No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTA78230.4274590094513083No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC73530.4017775912687549No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA70660.3860955337828128No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT69960.3822706417130708No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66250.3619987137434383No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG63910.3492126459674436No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA60830.33238312086057886No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT59190.3234219451543262No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA57040.3116740623686901No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT56270.3074666810919739No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC55880.30533566979597476No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT54670.29872407064684936No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC53110.29020002546285295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA52300.2857740789250086No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC51480.2812934910718823No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTG50310.27490045718388495No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC49110.26834349935004154No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48960.2675238796208111No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC48570.265392868324812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG48210.263425780974659No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGG48060.26260616124542857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG47290.25839877996871236No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG46530.2542460400072782No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT46480.2539728334308681No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGATCCCCGGGT45560.24894583242492146No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC44950.24561271219271774No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCC43680.23867326515190015No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG42580.232662720470877No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAG39800.2174724348224731No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA38690.21140724882616796No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA38410.20987729199827115No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG37520.20501421493817062No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT37490.20485029099232455No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA36830.20124396418371066No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT36040.1969273002764304No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA35810.19567055002494377No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC35340.19310240820668842No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA35340.19310240820668842No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC35230.19250135373858612No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG34580.18894966824525428No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCT34280.1873104287867934No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33890.1851794174907943No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC32960.18009777516956568No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC32900.1797699272778735No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA31960.1746336436413628No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC31770.1735954586510043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC31770.1735954586510043No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA31610.17272119760649182No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31370.17140980603972317No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT31330.17119124077859504No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT31030.16955200132013418No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG30760.1680766858075194No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC30710.16780347923110928No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC30580.1670931421324429No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA30270.1653992613587No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC30250.16528997872813597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30100.16447035899890555No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA29900.16337753269326497No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC29590.16168365191952208No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC29100.1590062274707027No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT28960.1582412490567543No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCA27170.14846045362127122No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGT27150.14835117099070716No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC26810.14649336627111817No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA26710.1459469531182979No Hit
ATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGA26590.14529125733491355No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT26030.14223134367911996No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA26010.14212206104855593No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTA25850.14124780000404347No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA25810.14102923474291534No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA25800.14097459342763333No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT25700.14042818027481305No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC25650.14015497369840288No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA25600.13988176712199274No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC25490.13928071265389044No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25080.13704041872732725No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTA24680.13485476611604613No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC24110.13174021114497053No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT24010.13119379799215022No Hit
CTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCA23880.13048346089348387No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC23750.12977312379481748No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA23740.12971848247953546No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC23670.12933599327256126No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23620.12906278669615112No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23610.1290081453808691No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC23220.12687713408486997No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23210.12682249276958796No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23110.12627607961676765No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22930.12529253594169115No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG22860.12491004673471696No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG22660.12381722042907638No Hit
ACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATT22470.12277903543871785No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT22310.1219047743942054No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA22290.12179549176364135No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGT22180.12119443729553903No Hit
GTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG22130.12092123071912887No Hit
GCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC22070.12059338282743673No Hit
AATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA21970.12004696967461642No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC21680.1184623715314376No Hit
GAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGC21600.11802524100918137No Hit
GTCTGGAGACTGGGTCATCACGATGTCCCCGTAGGCACCAGAGATCCAGA21170.11567566445205417No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGC20840.11387250104774721No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT20750.11338072921020896No Hit
GGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATC20370.11130435922949188No Hit
CCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGC20300.11092187002251769No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG20290.11086722870723566No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG20070.10966511977103102No Hit
GCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTG19880.1086269347806725No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC19850.1084630108348264No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC19580.10698769532221164No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCC18980.10370921640528993No Hit
GAATCGGTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCA18920.10338136851359776No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18820.10283495536077748No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCG18700.10217925957739314No Hit
GCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACTCTGGCTGGACTT18630.10179677037041894No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG18620.10174212905513691No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT18580.10152356379400881No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA18560.10141428116344474No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT18550.10135963984816271No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTA18500.10108643327175257No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA18460.10086786801062446No Hit
ACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGAC18360.10032145485780418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)