FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616265
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGG31690.5142268342352722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25340.4111867459615588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20900.3391398180977339No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20440.33167549674247276No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18500.30019553276593675No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15490.25135290824564105No Hit
GTATGGTAGCTCACCGTACACTTTTGGCCAGGGGGCCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15250.24745847971246135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14830.24064322977939684No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGGCCAAGCTGGAGATCAAACGAA14170.22993355131315263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13540.21971067641355585No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13230.21468037289153205No Hit
CTTTTGGCCAGGGGGCCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13160.21354449790268798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13110.2127331586249422No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12930.2098123372250574No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAAACTGGAGCCTGAAGATT12850.20851419438066418No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12460.2021857480142471No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12390.20104987302540303No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12340.20023853374765724No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11710.19001565884806051No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11640.18887978385921642No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG11570.18774390887037232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10940.17752103397077557No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10890.17670969469302977No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10560.17135485545990767No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10530.1708680518932602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10410.16892083762667034No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGC10230.16600001622678556No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGGAGTTGCTGCTAAGACTCTGA9990.16210558769360583No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9890.16048290913811428No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTTTGGTGATGGTGAGAGTGAAGTCTGTC9290.15074683780516498No Hit
GGCCAAAAGTGTACGGTGAGCTACCATACTGCTGACAGTAATACACTGCA9200.1492864271052226No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGCCCCCTGGCCAAAAGTG9030.14652787356088695No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCAGA9030.14652787356088695No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGCAGCAACTCCTTAGCCTGGTACC8950.1452297307164937No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGCCC8780.1424711771721581No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8630.14003715933892077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8630.14003715933892077No Hit
GACTTCACTCTCACCATCACCAAACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA8580.13922582006117498No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8580.13922582006117498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8550.13873901649452752No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA8330.1351691236724461No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8170.13257283798365962No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8050.13062562371706976No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7810.12673119518389003No Hit
GTCAGAGTCTTAGCAGCAACTCCTTAGCCTGGTACCAGCAGAAACCTGGC7700.12494624877284935No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7560.12267449879516117No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7350.11926687382862891No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7230.11731965956203905No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7170.11634605242874412No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6910.11212708818446611No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6720.10904399892903215No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6710.10888173107348299No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6660.10807039179573721No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT6380.10352689184036087No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6320.10255328470706596No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT6320.10255328470706596No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC6270.10174194542932019No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT6170.10011926687382862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)