FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60200.44528339847390586No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52660.38951202265175877No Hit
GGCTTACAGTTTCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT45300.33507205898451714No Hit
GGGTATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTATGCTGCATC41430.3064466976540518No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40570.3000855062472817No Hit
CTGTAAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGTCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTC39020.2886205682467077No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38400.28403459304647816No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA36380.26909318997476234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36260.26820558187149207No Hit
GTCTCTGGTGGCTCCATCAGTGGACATTACTGGGGCTGGATCCGGCTGTC34870.2579241213419451No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33340.2466071180252495No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32780.24246494687665504No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32650.24150337143144557No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32050.23706533091509435No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31530.23321902913425666No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31410.23233142103098642No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAA30420.22500865417900687No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29840.220718548346534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29660.2193871361916287No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC28830.21324784681067613No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28650.21191643465577076No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27550.20378002704246023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27230.20141307210040624No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27110.200525463997136No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26420.1954217174033321No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25910.19164938296443354No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT25040.18521422421572428No Hit
GCATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGAC24330.17996254293804198No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24140.17855716344119746No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23990.17744765331210965No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23790.1759683064733259No Hit
CTACTAGCATAGTAGCCCCCCCCATTGGGGACTCTCGCACAGAAATACAC22600.16716619278256264No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTGGACATTACTGGGGCTGGATCCGGCTGTCCCCCGGGAAGGGG22150.16383766239529923No Hit
GATTTCACTCTCACCATCAGCAGCCTACAGACTGAAGATTTTGCAACTTACTATTGTCAACAGGCTTACAGTTTC22030.16295005429202897No Hit
GTCTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACATGTC21560.1594735892208872No Hit
GAAATACACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGGTTCAGGGAGA20970.15510951604647516No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG20820.15400000591738736No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20810.15392603857544818No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20690.1530384304721779No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20630.1525946264205428No Hit
GCCTACAGACTGAAGATTTTGCAACTTACTATTGTCAACAGGCTTACAGT20400.15089337755594148No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19970.14771278185255646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19970.14771278185255646No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19420.1436445780459012No Hit
GTCTGTAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC19400.1434966433620228No Hit
GTTGTAGACAGCGATCCCAGTGTAAGACATATATCCGATCCACTCCAGCC18690.1382449620843405No Hit
ACCAGAGACAGTGCAGGTGAGCGACAGGGTCTCCGAAGGATTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAC18090.13380692156798932No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18070.13365898688411093No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18000.13314121549053662No Hit
GTGTAAGACATATATCCGATCCACTCCAGCCCCTTCCCGGGGGACAGCCG17960.13284534612277987No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17830.13188377067757043No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17680.13077426054848262No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17480.1292949137096989No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17330.1281854035806111No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT17310.1280374688967327No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17210.12729779547734082No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC16910.12507877521916524No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT16760.12396926509007744No Hit
GATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTGCTGATACCAGACTAACC16700.1235254610384423No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16560.12248991825129368No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA16290.12049280001893564No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15950.11797791039300327No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15910.11768204102524653No Hit
GTCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTC15900.11760807368330735No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC15850.11723823697361141No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15840.11716426963167223No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15770.11664649823809792No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15760.11657253089615872No Hit
GTCTTACACTGGGATCGCTGTCTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACATGTCCAAGAA15690.11605475950258443No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15670.11590682481870604No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15640.11568492279288849No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15500.11464938000573988No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15410.11398367392828719No Hit
GATATATGTCTTACACTGGGATCGCTGTCTACAACCCCTCCCTCAAGAGTCGAGTCACCATTTCAGTAGACATGT15400.113909706586348No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15390.1138357392444088No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG15390.1138357392444088No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15160.11213449037980751No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14980.11080307822490215No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14950.11058117619908459No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14940.11050720885714539No Hit
CACCATCAGCAGCCTACAGACTGAAGATTTTGCAACTTACTATTGTCAACAGGCTTACAGTTTCCCCACTTTCGG14680.10858405796672654No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14500.10725264581182116No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14490.10717867846988197No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA14440.10680884176018604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14410.10658693973436847No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14380.10636503770855092No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14270.10555139694721986No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAATCC14080.10414601745037531No Hit
GGTTAGTCTGGTATCAGCAGAAACCAGGGAAGGCCCCTAAGCTCCTGATC13980.10340634403098344No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGCTTAGGGGCCTTCCCTGGTTTCTG13680.10118732377280783No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13670.10111335643086863No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC13580.10044765035341598No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13560.10029971566953759No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGTCGCATCTGGAACCTGGGAACCAGAG13560.10029971566953759No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCA13550.1002257483275984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTA150.002480090668.1098564
CGTGCGT350.005051763356.9104970
GTACGCT200.00774808651.08050516
TACGTAT554.9671123E-448.28769370
CGAGACT3350.046.78449223
ACGAGAC3400.046.0879622
GCGACTA306.731319E-445.40321715
CGTATGC3150.044.98609541
TCGTATG3150.044.94391340
GTGGTAT3050.044.6871
TAGCGCT3500.043.89043829
GACTAGC3500.043.83355326
AGACTAG3500.043.8205725
ATCTCGT3150.043.63821837
GAGACTA3600.042.59229724
ATAATAC405.400804E-542.5686653
CTAGCGC3650.042.0617728
ACTAGCG3650.042.04307627
CACGAGA3650.041.99329821
CTTATAC3900.041.063341
Produced by FastQC (version 0.11.5)