FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110949
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT10700.964407069915006No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC2230.2009932491505106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.18567089383410396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.17305248357353378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.16944722349908517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.16854590848047302No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1730.15592749821990284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.13519725279182326No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1480.13339462275459896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.1306906776987625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12978936268015034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.12978936268015034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1310.11807226743819232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10996043227068293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.10545385717762215No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10455254215901No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1110.10004596706594922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.10004596706594922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA100.0033475102186.1040370
AGGGGGG1250.0119.1065870
AGTTATA200.001696171174.8744165
GAAACTT200.001880824772.9516663
AAGCGAG200.00288761465.4833855
AAGGGGG1350.064.5145869
CAAAGCG200.003127728864.1780753
AAAAGGG1250.061.61913767
CTTGAAA1600.058.56860457
AAAAAGG1300.058.3190566
GTATCAG150.004639392358.1155241
CTGATGT150.004639392358.1155241
ATCAAAA150.00465600258.0631211
TGAAAAA1900.057.6451259
AAAGGGG1450.055.1427368
AGCGTTG250.00640480953.5484457
AAGCGTT250.00670879452.9249356
TCTGCTT1700.052.8525353
TTGAAAA1900.052.28552658
AAAGCGA250.007175172752.0339454
Produced by FastQC (version 0.11.5)