FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110949
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19251.7350314108283988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.19107878394577688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.16133538833157576No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC1730.15592749821990284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.14871697807100562No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14330908795933267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.1388025128662719No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAG1480.13339462275459896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.12708541762431386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1320.11897358245680448No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.11626963740096802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.10905911725207076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10455254215901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGAC150.003298436563.33981331
GGGTAAG150.00443861458.76860411
CTTTGTA250.005868964454.743761
GGGGGTT806.9984683E-444.92758670
TAAATTA606.97061E-540.6952866
ATACCAT851.3973477E-831.1127937
GGGTGAG400.005661959829.3975641
TACCATC504.7445824E-429.3843068
CGAAGAA550.00788146927.46561244
CGGGACC600.001372620824.49796916
ACACCAA750.005026658523.9613870
GCTTCAC650.002158365222.66464820
GTATCAA16650.022.4233681
GATACCA3050.021.1952366
CGGGTGG700.003362423320.99825917
CCATCCC700.003371238720.98878710
ATCCCGT700.003371238720.98878712
TTTTCTG700.003371238720.9887875
GCAGAGT16600.020.8876369
AGAGTAC16650.020.6484311
Produced by FastQC (version 0.11.5)