FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819940
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT29900.3646608288411347No Hit
GTATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23690.28892357977412986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21780.26562919237993027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19920.24294460570285628No Hit
CTATTATACTTTTCACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC19770.2411152035514794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17030.20769812425299414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16680.2034295192331146No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15870.1935507476156792No Hit
ACTTATTACTGTGAAAAGTATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15790.19257506646827816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15450.1884284215918238No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15030.1833060955679684No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14340.17489084567163452No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13710.1672073566358514No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13500.16464619362392371No Hit
GCATAGATCAGGAGCTTAGGAACTTTCCCCGGTTTCTGCTGATACCAGGC12770.1557431031538893No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12570.15330390028538673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12430.15159645827743493No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12410.15135253799058468No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12360.15074273727345905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12300.15001097641290825No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11680.14244944752055028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11510.1403761250823231No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11430.13940044393492207No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10940.13342439690709076No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10890.13281459618996513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10350.12622874844500817No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9410.11476449496304608No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9390.11452057467619581No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9270.11305705295509429No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG9170.11183745152084298No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9130.11134961094714248No Hit
GATCAGGAGCTTAGGAACTTTCCCCGGTTTCTGCTGATACCAGGCCAAAT9110.11110569066029222No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9040.11025196965631633No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8940.10903236822206502No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8920.10878844793521478No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG8840.10781276678781375No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTTGCAACTTATTACTGTGAAAAGTATAATAGTTTC8800.10732492621411323No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCGAGATGGGACCCCT8400.10244652047710807No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8230.10037319803888088No Hit
GGTATCAGCAGAAACCGGGGAAAGTTCCTAAGCTCCTGATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCAT8210.10012927775203063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)