FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377905
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAAGGTGCCACCACTTGCATATGAGCAGCAGTAATAAT52800.38319042314237917No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35200.25546028209491944No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34710.2519041588498481No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACAGGAACCAGCAGTGATGTT34660.2515412891309633No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34370.2494366447614313No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32880.2386231271386634No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31900.23151088064852077No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAAGGTGCCACCACTTGCATATGAGCAG31750.23042227149186628No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29860.21670579611801974No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC29740.21583490879269618No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG29730.21576233484891919No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29270.21242393343517876No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC28820.20915810596521528No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG26720.19391757777205249No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26550.1926838207278441No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26170.1899260108643194No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25760.18695047916946378No Hit
GTAATAATCGGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG25760.18695047916946378No Hit
CTCCTGCACAGGAACCAGCAGTGATGTTGGGAGTTATAATTCTGTCTCCTGGTACCAGCAGAAGCCAGGCAAAGC25690.18644246156302502No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25410.18441039113726998No Hit
GTCGATCACCATCTCCTGCACAGGAACCAGCAGTGATGTTGGGAGTTATA23900.17345172562694816No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23810.17279856013295547No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23530.1707664897072004No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23080.16750066223723697No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22730.16496057420504318No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC22130.16060613757842523No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21520.1561791270080303No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21470.1558162572891455No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21000.15240528193162808No Hit
GATTATTACTGCTGCTCATATGCAAGTGGTGGCACCTTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT20790.1508812291123118No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20340.14761540164234835No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18820.13658416218824956No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18690.135640700919149No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18490.13418922204360967No Hit
GTTATAATTCTGTCTCCTGGTACCAGCAGAAGCCAGGCAAAGCCCCCAAA18330.13302803894317822No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18290.13273774316807035No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18250.13244744739296252No Hit
GAATTATAACTCCCAACATCACTGCTGGTTCCTGTGCAGGAGATGGTGAT18170.13186685584274677No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17860.12961706358566083No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17560.1274398452723519No Hit
CCCCTGAGGGCCACTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTTCTGCTGGTACCAGGAGA17210.12489975724015807No Hit
GACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCT16930.12286768681440303No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16780.12177907765774854No Hit
CTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTTCTGCTGGTACCAGGAGACAGAATTATAACT16710.12127106005130979No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16680.12105333821997886No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16630.12069046850109405No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC16070.11662632764958397No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15620.11336050017962052No Hit
GTCAGTAAGTGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT15360.1114735776414194No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15210.11038496848476491No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15180.11016724665343401No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15000.10886091566544864No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGAC14850.10777230650879414No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14820.10755458467746325No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14350.10414360931994586No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14310.10385331354483801No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC14220.10320014805084529No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14110.10240183466929868No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14080.10218411283796779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.10196639100663689No Hit
GTAAGTGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGC13860.10058748607487454No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13830.10036976424354364No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13830.10036976424354364No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC13790.10007946846843578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1200.045.9986240
GGTATCA16250.045.0912481
CGTATGC1300.042.53879541
GTATCAA42450.037.3990021
ATCTCGT1300.036.95895837
GACTGAT4150.036.6822770
CCCACGA1500.036.16988819
TCTCGTA1400.034.37711738
ACTTACG400.00282695833.90681516
ATGCCGT1650.033.6961544
CGACTGA3150.032.72806569
ATCTCCG1800.032.0219410
TACGTAT1252.3585271E-531.77003770
TATCAAC49950.031.5027332
ATCAACG51100.030.791533
TCAACGC51150.030.7603174
CAACGCA51700.030.4986695
AACGCAG52650.030.0127666
ACCGATT900.00566430729.416770
AGCGGAT900.00566430729.416770
Produced by FastQC (version 0.11.5)