FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377905
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAAGGTGCCACCACTTGCATATGAGCAGCAGTAATAAT48350.35089501816162944No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37340.2709911060631901No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACAGGAACCAGCAGTGATGTT33630.24406617292193583No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACCAAGGTGCCACCACTTGCATATGAGCAG31850.2311480109296359No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31610.22940623627898873No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC31520.22875307078499607No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30930.2244712081021551No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30880.22410833838327027No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG29780.216125204567804No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29400.21336739470427932No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29360.21307709892917145No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29310.21271422921028665No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27200.19740112707334687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27060.19638509186046932No Hit
CTCCTGCACAGGAACCAGCAGTGATGTTGGGAGTTATAATTCTGTCTCCTGGTACCAGCAGAAGCCAGGCAAAGC25490.1849909826874857No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24990.18136228549863742No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC24660.17896734535399755No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24380.17693527492824251No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23750.17236311647029365No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23550.17091163759475436No Hit
GTCGATCACCATCTCCTGCACAGGAACCAGCAGTGATGTTGGGAGTTATA23060.16735551434968302No Hit
GTAATAATCGGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG22740.1650331481488201No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22230.1613318770161949No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC22110.16046098969087128No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22100.16038841574709434No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21310.15465507418871402No Hit
GATTATTACTGCTGCTCATATGCAAGTGGTGGCACCTTGGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT21150.15349389108828257No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20270.1471073840359096No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20180.1464542185419169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19480.1413740424775293No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18990.13781791923245795No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAAGAC18600.13498753542515632No Hit
GTTATAATTCTGTCTCCTGGTACCAGCAGAAGCCAGGCAAAGCCCCCAAA18250.13244744739296252No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18200.13208457767407766No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17960.1303428030234305No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17900.12990735936076872No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17780.12903647203544513No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17460.1267141058345822No Hit
GAATTATAACTCCCAACATCACTGCTGGTTCCTGTGCAGGAGATGGTGAT17330.12577064456548165No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16820.1220693734328564No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16510.11981958117577046No Hit
CCCCTGAGGGCCACTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTTCTGCTGGTACCAGGAGA16280.11815038046890025No Hit
CTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGCTTCTGCTGGTACCAGGAGACAGAATTATAACT16190.11749721497490756No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16090.1167714755371379No Hit
GACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCT15840.11495712694271376No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15730.11415881356116714No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15580.11307020440451264No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15470.11227189102296603No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC15430.11198159524785815No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15400.11176387341652727No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15240.1106026903160958No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC15020.10900606355300256No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14810.1074820107336863No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14790.10733686284613235No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14650.10632082763325484No Hit
GTCAGTAAGTGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT14630.10617567974570088No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14600.10595795791437No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14340.10407103537616891No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCG13880.10073263396242846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.043.5094031
GTATCAA44050.038.7860531
TATCAAC49550.034.3265462
ATCAACG49550.034.182343
GCGCAAC400.00284467333.8636311
GACTGAT5600.033.71674370
TCAACGC50250.033.704944
CAACGCA51200.033.2118725
AACGCAG51850.032.9879236
CGCAGAG54300.031.3736258
ATGGTCG450.00444029530.9204344
AGAGTAC57000.030.12080811
CAGAGTA57300.029.90509410
GCAGAGT57800.029.6474749
TGGTATC5350.028.4989452
TACGTAT1455.7177967E-527.90351370
CGGTACC755.761496E-527.87516245
GTGGTAT5600.027.8458671
TATACTT1255.0495146E-927.0987725
GAGTACT27800.026.6796512
Produced by FastQC (version 0.11.5)