FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004691575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14358
Sequences flagged as poor quality0
Sequence length21-76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT549338.25741746761388No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC7455.188744950550215No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG3982.771973812508706No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2972.0685332218972RNA PCR Primer, Index 39 (95% over 23bp)
GAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC1621.1282908483075638No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT1491.0377489901100432RNA PCR Primer, Index 39 (96% over 25bp)
AGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG910.6337930073826439No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT910.6337930073826439No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCCGT750.5223568742164647No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATATCGTATGCCGT640.4457445326647165No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC570.3969912244045131No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA570.3969912244045131No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTA530.3691321911129684No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT410.28555509123833406RNA PCR Primer, Index 39 (95% over 22bp)
AGAGACAGCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCT370.25769605794678924No Hit
GAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT330.22983702465524447No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG310.21590750800947206Illumina PCR Primer Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG300.20894274968658588RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACA260.1810837163950411No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG240.1671541997492687RNA PCR Primer, Index 39 (96% over 30bp)
CAGCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGC230.1601894414263825No Hit
GCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG210.14625992478061012No Hit
GAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG200.1392951664577239No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA180.12536564981195153RNA PCR Primer, Index 39 (96% over 30bp)
AGAGACAGTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC170.11840089148906534No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATGTCGTATGCC160.11143613316617912No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT160.11143613316617912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)