FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944585
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTGTACAGCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT55100.5833249522276979No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACACTCAGGCTGTCATCCCATGCTGTACAGTAATACT41110.4352175823245129No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40670.4305594520344913No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40280.4264306547319722No Hit
GTAATACTCAGCTTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG39310.4161615947744247No Hit
TTACTGTACAGCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCC37390.39583520805433076No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37170.39350614290931996No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGCGTCTGGGGCCC36740.3889538792167989No Hit
GTACAGTAATACTCAGCTTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG36560.38704828046179013No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC36250.3837664159392749No Hit
GTATTACTGTACAGCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAG34610.3664042939491946No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACACTCAGGCTGTCATCCCATGCTGTA34180.36185203025667356No Hit
GTCCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGTGTCAC33780.357617366356654No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31770.33633818025905554No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGCGTCT30080.31844672528147283No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29630.3136827283939508No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT29460.3118829962364425No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28310.29970833752388615No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26560.28118168296130047No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26120.2765235526712789No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25960.2748296871112711No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25590.27091262300375296No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25240.26720729209123584No Hit
CCCCTGAGGGCCGCCGATTATTACTATAGATGAGGAGTTTGGGGGCCGTGCCTGGGATTTGGTGGTACCAGTTTA25100.26572515972622895No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24540.25979663026620153No Hit
GCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCC24140.255561966366182No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCAC23530.2491041039186521No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23510.2488923707236511No Hit
GTTTGGGGGCCGTGCCTGGGATTTGGTGGTACCAGTTTACAGCATGACGT22680.2401054431311105No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21990.23280064790357669No Hit
CAGTAATACTCAGCTTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA21500.2276131846260527No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21430.2268721184435493No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21430.2268721184435493No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21170.22411958690853653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21100.22337852072603312No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21060.2229550543360312No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21010.2224257213485287No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20280.21469745973099297No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20090.21268599437848368No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19950.21120386201347682No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19580.2072867979059587No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18970.20082893545842884No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18910.2001937358734259No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18810.199135069898421No Hit
GTACAGCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG18690.1978646707284151No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACA18650.1974412043384132No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17510.18537241222335735No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT17460.1848430792358549No Hit
ATCCCATGCTGTACAGTAATACTCAGCTTCATCCTCAGACTGGAGCCCAC17350.1836785466633495No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17130.18134948151833874No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16580.1755268186558118No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16570.17542095205831132No Hit
ATGCTGTACAGTAATACTCAGCTTCATCCTCAGACTGGAGCCCACTGATG16210.1716097545482937No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16200.17150388795079322No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16200.17150388795079322No Hit
AGTCTGTCCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGT15740.16663402446577066No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15690.16610469147826823No Hit
GTTTACAGCATGACGTCCGATGTTGGAATTGCTTCCAGAACAAGAGATGGTGACACTCTGCCCGGGGGCCCCAGA15640.1655753584907658No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15380.16282282695575306No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAAGCTGAGTATTACTGTACAG15280.16176416098074817No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACACTCAGG14730.15594149811822125No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGTGTCACCATCTCTTGTTCTGG14730.15594149811822125No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14700.15562389832571977No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14510.15361243297321045No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14330.15170683421820164No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14150.14980123546319285No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14080.1490601692806894No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14010.14831910309818597No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13910.1472604371231811No Hit
GCCCCCAAACTCCTCATCTATAGTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13830.14641350434317718No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13780.14588417135567472No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13720.14524897177067178No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGACGTCATGCTGTAAACTGGTACCACCAAATCCCAGGCACGGCCCC13680.14482550538066982No Hit
GGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCC13310.14090844127315172No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13300.14080257467565122No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13270.14048497488314973No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13220.1399556418956473No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13030.13794417654313798No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG13020.1378383099456375No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12690.13434471222812133No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12470.13201564708311056No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12390.13116871430310664No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12370.13095698110810566No Hit
GCTGTAAACTGGTACCACCAAATCCCAGGCACGGCCCCCAAACTCCTCAT12330.13053351471810373No Hit
GAGTGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGACGTCATG12270.12989831513310077No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12080.12788684978059148No Hit
GTCTGAGGATGAAGCTGAGTATTACTGTACAGCATGGGATGACAGCCTGA12040.12746338339058952No Hit
GTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11820.12513431824557875No Hit
AGTCAGGACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATG11700.12386391907557287No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11680.1236521858805719No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11530.12206418691806453No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC11530.12206418691806453No Hit
GGATTTGGTGGTACCAGTTTACAGCATGACGTCCGATGTTGGAATTGCTTCCAGAACAAGAGATGGTGACACT11520.12195832032056407No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11270.11931165538305183No Hit
GTGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGACGTCATGCT11210.11867645579804888No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11100.1175119232255435No Hit
CTATAGATGAGGAGTTTGGGGGCCGTGCCTGGGATTTGGTGGTACCAGTT11080.11730019003054251No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10940.11581805766553567No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCCG10910.11550045787303419No Hit
GCTTGGTCCCTCCGCCGAATGCCACACTCAGGCTGTCATCCCATGCTGTA10830.11465352509303027No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAG10820.11454765849552978No Hit
GTCAGGGACCCCTGAGGGCCGCCGATTATTACTATAGATGAGGAGTTTGGGGGCCGTGCCTGGGATTTGGTGGTA10820.11454765849552978No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10570.11190099355801754No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10500.11115992737551411No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10120.1071369966704955No Hit
GATGAGGAGTTTGGGGGCCGTGCCTGGGATTTGGTGGTACCAGTTTACAG10090.10681939687799405No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC10090.10681939687799405No Hit
CATCTATAGTAATAATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC10070.10660766368299306No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10060.10650179708549257No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC10050.10639593048799208No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGC10020.10607833069549062No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC9990.10576073090298914No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9960.10544313111048767No Hit
GAACAAGAGATGGTGACACTCTGCCCGGGGGCCCCAGACGCTGAGGGTGG9850.1042785985379823No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC9830.1040668653429813No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT9630.10194953339297151No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9620.10184366679547104No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATGCTGGAGATTGTCCTGAGT9620.10184366679547104No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG9560.10120846721046808No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGCGTCTGGGG9530.10089086741796663No Hit
GTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9480.10036153443046417No Hit
ACTGTACAGCATGGGATGACAGCCTGAGTGTGGCATTCGGCGGAGGGACC9450.10004393463796271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)