FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412949
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCATCAGTGTGATAGCTCACCGTACACTTTTGGCCAGGGGA41500.29371194572486337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34610.24494868533825354No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34300.2427546924906702No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32540.23029847503342302No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26210.18549855656502817No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25550.18082747501856047No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24820.1756609757323159No Hit
CTCCTGTAGGGCCAGTCAGAGTGTCAGAGATGAATTCTTAGTCTGGTACC24110.17063602437172185No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACAA23810.1685128054869638No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22640.1602322518364074No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21410.1515270544088994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20230.14317572679551774No Hit
GTGTATTACTGTCATCAGTGTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAA19660.13914161091447746No Hit
GTGTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCAT19540.13829232336057423No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19290.13652297428994253No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19040.1347536252193108No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT18830.13326737199998018No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18740.13263040633455278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18690.13227653652042642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18540.1312149270780474No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCA18520.13107337915239686No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT18470.1307195093382705No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18440.13050718744979473No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18270.12930403008176516No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17460.12357133909291843No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17020.12045728472860663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15930.11274292278065237No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15770.11161053937544808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15760.11153976541262282No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15680.11097357371002067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15580.11026583408176799No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15420.1091334506765637No Hit
GGCCAAAAGTGTACGGTGAGCTATCACACTGATGACAGTAATACACTGCA14830.10495778686987287No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT14490.10255147213381374No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14450.10226837628251267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCC14260.10092367098883258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)