FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412949
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCATCAGTGTGATAGCTCACCGTACACTTTTGGCCAGGGGA39470.2793448312713339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36730.2599527654572104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34320.24289624041632074No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29360.20779235485498768No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28990.20517371823045275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26200.1854277826022029No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26090.18464926901112497No Hit
CTCCTGTAGGGCCAGTCAGAGTGTCAGAGATGAATTCTTAGTCTGGTACC25420.17990741350183198No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23790.16837125756131324No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACAA23740.1680173877471869No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21090.1492622875984908No Hit
GTGTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCAT21020.14876686985871396No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20670.14628978115982955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19640.13900006298882692No Hit
GTGTATTACTGTCATCAGTGTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAA19590.1386461931747006No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19460.1377261316579721No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19200.1358860086245151No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19190.13581523466168985No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCT18320.1296578998958915No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18240.12909170819328936No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCA17890.12661461949440497No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16890.11953722321187814No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.11592775110778947No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16280.11522001147953678No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16060.11366298429738086No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15770.11161053937544808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15610.1104781559702438No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15460.10941654652786477No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15300.10828416312266048No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15010.1062317182007277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14980.10601939631225189No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14950.1058070744237761No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14680.10389617742749384No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT14360.10163141061708526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGT300.002425843268.4871870
GGTATCA14300.040.1844561
TACGTAT2051.2732926E-1136.74921870
GTATCAA37550.035.914031
ATCGACT400.002548823134.63062342
TATCAAC41500.032.1641542
ATCAACG42400.031.2387453
TCAACGC42650.031.2124084
GTGGTAT3850.031.0591661
CAACGCA43400.030.673025
AACGCAG44450.029.8748346
CGCAGAG45850.028.8188348
ACGCAGA46200.028.6713567
AGAGTAC46750.028.12001211
GCAGAGT47150.027.953849
AACTGTG24500.025.43809570
GTACGTA2001.0550139E-925.27443169
AGTACGG41200.024.49851613
GAGTACG41400.024.37843912
ACGATTA851.4859985E-424.24899740
Produced by FastQC (version 0.11.5)