FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636784
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39650.6226601170883691No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33970.5334618960275384No Hit
GATCTATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA32780.5147742405588079No Hit
GAGTTACAGTACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT30160.473629990703284No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25210.39589562551822904No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23660.37155456167240386No Hit
GTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT23270.365430035930551No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22650.3556936103922209No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21380.3357496419508028No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21080.3310384683032237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21010.3299391944521219No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20870.3277406467499184No Hit
GCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20510.32208723837282344No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19620.30811075655167214No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18410.28910902283976986No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTC18180.28549712304329256No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17620.2767029322344783No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17520.27513254101861856No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGA17490.27466142365386065No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17410.2734051106811729No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGG17110.26869393703359384No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16730.262726450413327No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16170.25393225960451266No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCGTACGTGGACGTTCGGCCA16030.2517337119023091No Hit
GTAATATACAGCCGTGTCCTCAGTTCTCAGACTGCTCATTTGAAGATACA15950.25047739892962134No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15940.25032035980803535No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15470.24293952109349484No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15410.24199728636397902No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAGATGTGACAT14850.23320309555516472No Hit
CCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAG14690.23069046960978917No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14670.23037639136661725No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14490.2275496871780698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13800.21671398778863793No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13080.20540717103444808No Hit
GATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT13040.20477901454810424No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12880.20226638860272872No Hit
GATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTACC12690.1992826452925953No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA12460.19567074549611801No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12420.19504258900977411No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12270.19268700218598458No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAA12050.18923214151109324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11870.18640543732254578No Hit
CATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTACCCGTACGTGGACGTT11830.18577728083620193No Hit
GTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTAC11100.17431342496042615No Hit
GTACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA11030.17321415110932434No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10690.1678748209754014No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10680.16771778185381545No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10540.16551923415161185No Hit
ATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA10440.16394884293575215No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10400.16332068644940828No Hit
GCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10300.16175029523354859No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10260.1611221387472047No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10130.1590806301665871No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10080.15829543455865724No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10040.15766727807231337No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9940.15609688685645368No Hit
CCCCTGATCTATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTT9920.15578280861328175No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9910.15562576949169576No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9870.1549976130053519No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9840.15452649564059398No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9820.15421241739742203No Hit
CCTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGA9660.15169979145204654No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9470.1487160481419131No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9400.14761677429081133No Hit
GTATATTACTGTGTGAAAAGCACTTGTACTGTTATCAGTTGCTATCGGGG9270.14557526571019372No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9030.14180632679213045No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9020.1416492876705445No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8940.14039297469785672No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8910.1399218573330988No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8880.1394507399683409No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8830.13866554436041106No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8820.1385085052388251No Hit
TCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGT8710.13678107490137942No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8680.1363099575366215No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCC8670.13615291841503555No Hit
CCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG8610.13521068368551975No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8590.1348966054423478No Hit
AGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATG8470.13301213598331615No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8390.1317558230106284No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8370.1314417447674565No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8350.13112766652428454No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8340.13097062740269858No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8310.13049951003794066No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8220.12908615794366693No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8150.12798688409256515No Hit
GCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTG8140.12782984497097918No Hit
ACCTTGATGGGACCCCACCTTCCAAACTGGATGCAGCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTG8010.12578833639036155No Hit
CACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAAC7970.12516017990401768No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7970.12516017990401768No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7940.12468906253925978No Hit
TACGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGT7800.1224905148370562No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7780.12217643659388427No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7780.12217643659388427No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTA7610.1195067715269228No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7610.1195067715269228No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7530.11825045855423504No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTG7450.11699414558154726No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7380.11589487173044549No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7320.11495263700092967No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTT7200.11306816754189804No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATT7150.11228297193396819No Hit
GTTACAGTACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT7130.11196889369079624No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC7090.11134073720445237No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7080.1111836980828664No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC7070.11102665896128043No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7070.11102665896128043No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7020.1102414633533506No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7010.11008442423176461No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG6970.10945626774542074No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA6960.10929922862383477No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGTACGGGTACTGTAACTCTGTTGA6890.10819995477273298No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCAT6840.10741475916480313No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGT6800.10678660267845926No Hit
ACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT6770.10631548531370134No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6750.10600140707052941No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG6720.1055302897057715No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATC6680.10490213321942762No Hit
ATGCAGCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTCGTTAATGCT6650.10443101585466971No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6440.10113319430136436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG159.975821E-485.6709469
GTCGTAT301.1321572E-768.98778539
GCCGTTA150.002448414568.3268415
CCGTTAA150.002448414568.3268416
TACCGCG150.00244917768.321478
TGTCGTA309.195377E-657.4215338
GGTTGGA350.00607643154.30947570
ATATAGG200.006997925752.40988550
TGATAGT250.007555244551.4025669
GTACCGC200.007650681351.2411087
CGGCCGT200.007650681351.24110813
ACCGCGG200.007650681351.2411089
GACTATA200.007650681351.2411087
CGTATGC707.2759576E-1249.3866441
GATGTCG352.317074E-549.121236
ACGAGAC800.047.0042322
TGAGCGA751.8189894E-1145.73439431
GTGGTAT1450.044.786961
CGAGACA1000.044.4438623
AGACACT851.8189894E-1244.2531925
Produced by FastQC (version 0.11.5)