FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636784
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39130.6144940827658987No Hit
GATCTATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA31180.4896479811050529No Hit
GAGTTACAGTACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29340.4607527827332345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26890.42227819794467203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24700.38788663031734466No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24530.3852169652503832No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24000.3768938918063268No Hit
GTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT23270.365430035930551No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21900.3439156762732732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21020.3300962335737079No Hit
GCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18890.2966469006758964No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18810.29539058770320864No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17740.2785874016935099No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17570.2759177366265484No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTC16930.2658672328450463No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16750.2630405286564989No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16650.26147013744063924No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16610.26084198095429534No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGA16520.2594286288600216No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16500.2591145506168497No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16270.2555026508203724No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15790.24796477298424582No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15710.2467084600115581No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGG15690.24639438176838613No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAA15040.23618683886529815No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCGTACGTGGACGTTCGGCCA14850.23320309555516472No Hit
GTAATATACAGCCGTGTCCTCAGTTCTCAGACTGCTCATTTGAAGATACA14770.23194678258247697No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14750.231632704339305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14690.23069046960978917No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14300.22456594386793638No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14220.22330963089524863No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14190.22283851353049072No Hit
CCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAG13980.21954069197718537No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13880.21797030076132567No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13600.2135732053569185No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAGATGTGACAT13100.20572124927762006No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA12640.19849744968466546No Hit
GATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTACC12490.19614186286087593No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12230.19205884569964068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12120.19033141536219503No Hit
GATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT11830.18577728083620193No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11340.1780823638784894No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11340.1780823638784894No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11190.17572677705469986No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11080.1739993467172542No Hit
CATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTACCCGTACGTGGACGTT10970.17227191637980852No Hit
GTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTAC10810.16975929043443302No Hit
CCCCTGATCTATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTT10730.16850297746174525No Hit
GCTCCTGGGGCTCCTGCTCCTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10310.16190733435513455No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10290.1615932561119626No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10280.16143621699037664No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10220.16049398226086084No Hit
ATGCTGCATCCAGTTTGGAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA10220.16049398226086084No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10100.1586095128018292No Hit
GTACCCGTACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10020.15735319982914142No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA9940.15609688685645368No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9800.1538983391542501No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9700.1523279479383904No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9630.15122867408728863No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9520.14950124374984297No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9240.1451041483454358No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9180.14416191361591998No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8910.1399218573330988No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8730.13709515314455137No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8690.1364669966582075No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8640.13568180105027763No Hit
CCTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGA8590.1348966054423478No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTCCTCTGGCTCC8480.13316917510490212No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8460.1328550968617302No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8440.13254101861855824No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTA8420.13222694037538632No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8400.13191286213221437No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8380.13159878388904245No Hit
CCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG8330.13081358828111259No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8290.1301854317947687No Hit
AGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCCCCTGATCTATG8270.12987135355159676No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8270.12987135355159676No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8170.12830096233573707No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8010.12578833639036155No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8000.1256312972687756No Hit
GTATATTACTGTGTGAAAAGCACTTGTACTGTTATCAGTTGCTATCGGGG7980.12531721902560364No Hit
CACCATCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAAC7910.12421794517450187No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7850.12327571044498606No Hit
TCAGTACTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGT7740.12154828010754039No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7710.12107716274278248No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7560.11872157591899293No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7500.11777934118947712No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7480.11746526294630517No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7360.11558079348727354No Hit
GCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTG7310.11479559787934371No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA7280.11432448051458578No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7220.11338224578506997No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7210.113225206663484No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7210.113225206663484No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7150.11228297193396819No Hit
TACGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGT7090.11134073720445237No Hit
CACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC7020.1102414633533506No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATTCTG6900.10835699389431896No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6880.10804291565114701No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6780.1064725244352873No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6710.10537325058418553No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6700.10521621146259957No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6690.10505917234101358No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCAT6690.10505917234101358No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGTACTCTGCAACCTGAAGATT6680.10490213321942762No Hit
ACCTTGATGGGACCCCACCTTCCAAACTGGATGCAGCATAGATCAGGGGCTTAGGGGCTTTCCCTGGTTTCTG6610.10380285936832584No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT6590.1034887811251539No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG6590.1034887811251539No Hit
GAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAG6570.10317470288198195No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC6550.10286062463881003No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGTACGGGTACTGTAACTCTGTTGA6510.10223246815246614No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6490.10191838990929421No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6480.10176135078770823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)