FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435918
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23710.543909634380778No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC8390.19246739065604082No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC7860.18030914070995002No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC6820.15645144270252662No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT6480.1486518106616382No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6150.14108157956312883No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT5770.13236434375272416No Hit
GATTATTACTGTGCAGCATGGGATAATAGCCTGAATGGTTGTGTCTTCGG5730.13144673998320783No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC5730.13144673998320783No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA5570.1277763249051427No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT5120.1174532824980845No Hit
GTAATAATCAGCGTCATCCTCAGACTGGAGGCCACTGATGACCAGGGAGG5110.11722388155570543No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG4910.11263586270812401No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC4810.1103418532843333No Hit
GTCCTCAGGGGTCCCTGCCCGATTCTCTGGGTCAAGGTCTGGCACCTCAG4790.10988305139957515No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4720.10827724480292164No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG4700.10781844291816352No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC4650.10667143820626815No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC4420.10139521653154951No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.10070701370441229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT400.00637973353.63606370
GCGTATC200.00985150348.06284320
GTCCGGA200.00986045448.05179617
TCGTCCG200.00988285748.02420415
GTCGTCC200.009900806548.0021514
AGTCGTC200.009900806548.0021513
ATATTAC200.009900806548.002151
GAGTCGT200.0099097947.9911312
ACGGGTA605.199872E-737.36932816
GTAGTTT1355.5034434E-737.08172270
CAGGTAT704.235335E-836.5730671
AGGTATC757.78673E-834.127032
GGTAGTT1001.0593267E-634.11453669
CGAAATT751.0995991E-633.9572248
CCAACCG751.3436857E-633.09759543
GTATCAA32500.032.6906971
ATGGCAA3950.032.58970
CGGGTAC502.8738746E-432.0345317
GTAGAAC400.003754373431.994093
AAGGCGC502.897209E-431.99041710
Produced by FastQC (version 0.11.5)