FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025755
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC107850.5323940950411081No Hit
GTCTTGGCTGACGCAGCCGACCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCTCCTGTACTGGCAGCAA102660.506774017588504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG101300.5000604712810779No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT93500.4615563086355458No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC93070.4594336432589331No Hit
CTCCTGTACTGGCAGCAACTCCAACCTCGGGGCCGGTTATGATGTGCATTGGTACCAGCAACTTCCAGGGACAGC92300.45563259130546385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA88220.4354919523831855No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC78470.3873617490762703No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG77870.3843998904112294No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC76300.37664969357103895No Hit
GTTATGATGTGCATTGGTACCAGCAACTTCCAGGGACAGCCCCCAAGTTCCTCATTTATGGTGACACCAATCGGC75440.3724043628178136No Hit
GCCTTGGGCTGACCGAGGACGGTGACCGTGGTCCCATTTCCGAAGACGTAAGACCTCAGTCTGCTGTCATAGGA71980.3553243111827442No Hit
GTCCTGGGCGCAGTCTTGGCTGACGCAGCCGACCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCTC69250.3418478542568079No Hit
GTCCTATGACAGCAGACTGAGGTCTTACGTCTTCGGAAATGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGC65710.3243728881330664No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC64390.3178567990699764No Hit
GGGCAAAGGGTCACCATCTCCTGTACTGGCAGCAACTCCAACCTCGGGGCCGGTTATGATGTGCATTGGTACCAG59400.29322400783905256No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG57250.28261068095598924No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT55330.27313273322785825No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCGCAGT53400.2636054211886433No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT48740.24060165222349197No Hit
CTGCACAGGGTCCTGGGCGCAGTCTTGGCTGACGCAGCCGACCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCAC48450.23917008720205551No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT47330.23364128436064577No Hit
GACTATTACTGCCAGTCCTATGACAGCAGACTGAGGTCTTACGTCTTCGG46380.2289516748076643No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG46240.2282605744524881No Hit
GGATGAGGCTGACTATTACTGCCAGTCCTATGACAGCAGACTGAGGTCTTACGTCTTCGGAAATGGGACCACGGT46110.2276188384083959No Hit
GACCATCACTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCCAGTCCTATGACAGCAGACTGAGGTCTTA45890.22653282356454754No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCGTGGTC45310.22366969352167462No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG45220.2232254147219185No Hit
GTCATAGGACTGGCAGTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAG44890.22159639245614599No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCCAGTCCTAT42810.21132861575067075No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG42010.2073794708639495No Hit
CTATTACTGCCAGTCCTATGACAGCAGACTGAGGTCTTACGTCTTCGGAA41860.20663900619768927No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT39510.1950383930929456No Hit
GCACAGGGTCCTGGGCGCAGTCTTGGCTGACGCAGCCGACCTCAGTGTCT38130.18822611816335144No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC38040.1877818393635953No Hit
GCCCCCAAGTTCCTCATTTATGGTGACACCAATCGGCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGT37960.18738692487492317No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC37800.18659709589757892No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA37780.1864983672754109No Hit
GGGTCACCATCTCCTGTACTGGCAGCAACTCCAACCTCGGGGCCGGTTATGATGTGCATTGGTACCAGCAACTTC34930.17242953861646645No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT34370.16966513719576157No Hit
GGGTCCTGGGCGCAGTCTTGGCTGACGCAGCCGACCTCAGTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCTC33600.16586408524229238No Hit
CATTTATGGTGACACCAATCGGCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC33260.16418569866543586No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC32780.1618162117334031No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC32780.1618162117334031No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC32140.1586568958240261No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC32110.15850880289077404No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT30910.1525850855606922No Hit
GCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG30810.15209144244985204No Hit
GTCTGGCACCTCAGCCTCCCTGACCATCACTGGGCTCCAGGCTGAGGATG30800.15204207813876802No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30750.15179525658334794No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT30740.15174589227226393No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG30130.14873466929613896No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC29900.1475992901412066No Hit
AGCCAAGACTGCGCCCAGGACCCTGTGCAGTGAGCGAGGAGAGTGAGGAG29880.14750056151903856No Hit
TCCAGGGACAGCCCCCAAGTTCCTCATTTATGGTGACACCAATCGGCCCTCGGGGGTCCCTGACCGATTCTCTGG29850.14735246858578654No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT29650.14636518236410623No Hit
CTTCCAGGGACAGCCCCCAAGTTCCTCATTTATGGTGACACCAATCGGCCCTCGGGGGTCCCTGACCGATTCT29450.1453778961424259No Hit
CTCCAACCTCGGGGCCGGTTATGATGTGCATTGGTACCAGCAACTTCCAG29280.14453870285399764No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT28520.14078701521161247No Hit
GCTCCAGGCTGAGGATGAGGCTGACTATTACTGCCAGTCCTATGACAGCA28230.139355450190176No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC28120.13881244276825183No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA27840.1374302420578994No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT27480.13565312685887485No Hit
GTGATGGTCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC27440.13545566961453875No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27230.13441901908177445No Hit
GTCTGGGGCCCCAGGGCAAAGGGTCACCATCTCCTGTACTGGCAGCAACT27010.1333330042379261No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG26970.13313554699359006No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC26970.13313554699359006No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCGCAGTCTTGG26830.13244444663841382No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC26640.13150652472781754No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG25880.12775483708543234No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA25740.12706373673025612No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG25190.12434869962063527No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG25060.12370696357654307No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG24690.1218804840664345No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24420.12054764766716607No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA24330.12010336886740994No Hit
TGGTGACACCAATCGGCCCTCGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGAC23340.1152163020700924No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGTCAGGGAGG23190.11447583740383215No Hit
GTGTCTGGGGCCCCAGGGCAAAGGGTCACCATCTCCTGTACTGGCAGCAA23040.11373537273757191No Hit
CTCACTGCACAGGGTCCTGGGCGCAGTCTTGGCTGACGCAGCCGACCTCA22910.11309363669347972No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT22870.11289617944914365No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG22850.11279745082697562No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC22730.11220507909396743No Hit
GACCGAGGACGGTGACCGTGGTCCCATTTCCGAAGACGTAAGACCTCAGT22640.11176080029421129No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA22480.11097097131686703No Hit
GCATTGGTACCAGCAACTTCCAGGGACAGCCCCCAAGTTCCTCATTTATGGTGACACCAATCGGCCCTCGGGGGT22290.11003304940627075No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22230.10973686353976667No Hit
GGCTGAGGATGAGGCTGACTATTACTGCCAGTCCTATGACAGCAGACTGAGGTCTTACGTCTTCGGAAATGGGAC22170.10944067767326256No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG21840.10781165540749003No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC21840.10781165540749003No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG21770.10746610522990195No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG21580.10652818331930564No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT21570.10647881900822163No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGTCAGGGAGGCTGAGGTGCC21510.10618263314171753No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGAC21400.10563962571979335No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC21330.10529407554220524No Hit
GTCTTACGTCTTCGGAAATGGGACCACGGTCACCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT21250.10489916105353313No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG21030.10381314620968478No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC20970.1035169603431807No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG20750.10243094549933235No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT20600.10169048083307211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.043.683261
GTGGTAT5950.040.5475461
TGGTATC6200.038.8981482
GCGACGT558.216422E-637.0644335
GTATCAA49400.036.6282231
AATGGTA12450.036.58823470
TATCAAC53150.033.779222
CAAACGA1303.6379788E-1232.58962249
CGAAATA450.003452321332.55232660
ATCAACG55750.032.135053
TCAACGC56000.031.9892234
CAACGCA57150.031.3455225
AACGCAG57600.031.1572276
AACGAAC1401.0913936E-1130.45369351
AAACGAA1401.0913936E-1130.35188950
CGCAGAG60650.029.701528
ACGCAGA60700.029.6763237
AGAGTAC61550.028.8849911
GCAGAGT62550.028.5288479
CAGAGTA62700.028.40790210
Produced by FastQC (version 0.11.5)