FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976837
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGACTGGCAATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG43180.44203894815614064No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC42790.43804647039372996No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41410.4239192413882767No Hit
CTCTACCACTCAGGCCGCTGTCATAGGACTGGCAATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGG41180.42156470322070105No Hit
GCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACACTCTACCACTCAGGCCGCTGTCATAGGA38100.39003436602012415No Hit
GACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACACTCTACCACTCAGG32290.3305566844826721No Hit
CTCCTGCACTGGGACTACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGC30780.3150986295564152No Hit
GTCCTATGACAGCGGCCTGAGTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCCAAGG30170.30885398485110616No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29900.3060899617848218No Hit
GTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCC29830.3053733632120814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27120.27763076132456077No Hit
GTAATAAAGGGCGGTATCTTCAGTTCTCAGATCATTCATTTGCAAAAACAGGGAGCTTTTGCTGTTGTCCCTGGA26360.26985054824909377No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTC24640.2522426976046157No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24640.2522426976046157No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23320.23872969594722557No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22230.22757123245741104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21370.21876730713517198No Hit
GGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCATCTATATTAACAACAATCGG21020.21518431427147008No Hit
GATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCAT20620.21108946528438213No Hit
GTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20370.2085301846674522No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19900.2037187371076239No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19660.20126182771537113No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19420.19880491832311842No Hit
GAGTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT19100.19552903913344805No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCCAGT19100.19552903913344805No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19000.1945053268866761No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18280.1871345987099178No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18110.18539428789040546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17730.18150418135267193No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17700.18119706767864033No Hit
CTTTATTACTGTGCAAAAGGAGGATATCGTTATGGTTCGGGGAGTATCCT17640.18058284033057717No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17140.17546427909671727No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16720.17116468766027493No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16690.17085757398624335No Hit
CTACCACTCAGGCCGCTGTCATAGGACTGGCAATAATAATCAGCCTCATC16240.16625086887576945No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGACTACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG15420.1578564284522392No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15320.15683271620546724No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG14920.15273786721837931No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCCAGTCCTAT14730.15079281394951258No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGACTAC14390.14731219231048784No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG14370.14710744986113342No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14240.14577662394032986No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13770.14096517638050157No Hit
ATATTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGG13760.14086280515582436No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13620.1394296080103436No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13600.1392248655609892No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT13430.13748455474147683No Hit
ATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13250.13564187269728725No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13230.13543713024793289No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACA12930.13236599350761694No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT12910.13216125105826254No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12600.1289877430932694No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12550.1284758869698834No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12540.1283735157452062No Hit
ACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCAT12420.12714506104907985No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGGTAGTCCCAGTGCAGGAGA12260.12550712145424467No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12250.12540475022956749No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12000.12284546961263752No Hit
TTCCAGGAACAGCCCCCAAACTCCTCATCTATATTAACAACAATCGGCCC11930.12212887103989714No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11860.12141227246715676No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11850.12130990124247956No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11630.1190577342995812No Hit
GCCCCCAAACTCCTCATCTATATTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11400.11670319613200565No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11290.11557711266055647No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT11180.1144510291891073No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11160.11424628673975289No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCAGCTTGGTC10720.10974195285395619No Hit
GTTTGGGGGCTGTTCCTGGAATCTGCTGGTACCAGTGTACATCATAACCT10650.1090253542812158No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTTCATGATCATCTCATGCACTGGGTCCGTCAAGTTACGGGGAAGGGT10620.10871824060718421No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10550.10800164203444382No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10530.10779689958508942No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC10280.10523761896815947No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10260.10503287651880508No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10190.10431627794606468No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10190.10431627794606468No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10180.1042139067213875No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9970.10206411100316633No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9970.10206411100316633No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9960.10196173977848914No Hit
GATTATTATTGCCAGTCCTATGACAGCGGCCTGAGTGGTAGAGTGTTCGG9930.10165462610445754No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9910.10144988365510316No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGG9830.10063091385768556No Hit
CATCATAACCTGCCCCGATGTTGGAGGTAGTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA9770.10001668650962238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACG100.009594999131.040670
CGAGACG1500.050.03803623
ATGCCGT1550.049.24315644
TACCCAT400.00902626249.1402370
CACGAGA1550.048.4214321
CGTATGC1500.048.3677141
TCGTATG1500.048.31511740
ATAGAGC306.662964E-445.496113
GGTATCA6100.045.316611
ATCTCGT1550.044.3600837
CTTATAC1800.043.6071361
GCTACAT1650.043.5731132
CCGTCTT1800.042.58115847
TATACAC1900.041.305683
TATGCCG1850.041.20629543
ATCTCCG1850.040.569310
GTATCAA17350.040.520051
TCTCGTA1700.040.49818438
GCCGTCT2000.040.00852646
ACGAGAC1900.039.5016922
Produced by FastQC (version 0.11.5)