FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976837
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39230.4016023144086475No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37510.38399446376416946No Hit
CTCTACCACTCAGGCCGCTGTCATAGGACTGGCAATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGTGATGG33780.3458099969595746No Hit
GTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCC33420.3421246328711955No Hit
GTCATAGGACTGGCAATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAG32810.33587998816588643No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32090.32850925998912817No Hit
CTCCTGCACTGGGACTACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGC31630.32380018365397706No Hit
GTCCTATGACAGCGGCCTGAGTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCCAAGG30130.3084444999523974No Hit
GCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACACTCTACCACTCAGGCCGCTGTCATAGGA29790.3049638783133727No Hit
GACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACACTCTACCACTCAGG25230.2582825998605704No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24750.25336878107606486No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24440.25019527311107176No Hit
GTAATAAAGGGCGGTATCTTCAGTTCTCAGATCATTCATTTGCAAAAACAGGGAGCTTTTGCTGTTGTCCCTGGA24350.249273932088977No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22970.23514670308352364No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22300.2282878310301514No Hit
GTCCTGGGCCCAGTTTGTCCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG21580.22091710285339317No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21570.22081473162871593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21360.21866493591049477No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21140.21641276896759645No Hit
GGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCATCTATATTAACAACAATCGG21130.2163103977429192No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT21100.21600328406888764No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20530.21016812426228737No Hit
GATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCAT20160.20638038894923105No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTC19110.19563141035812529No Hit
CTGCACAGGGTCCTGGGCCCAGTTTGTCCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC19000.1945053268866761No Hit
GAGTGGTAGAGTGTTCGGCGGAGGGACCAAGCTGACCATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18880.1932768721905497No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18780.19225315994377773No Hit
GGCCATCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCCAGT18570.19010336422555657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17690.18109469645396314No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17520.17935438563445077No Hit
CTTTATTACTGTGCAAAAGGAGGATATCGTTATGGTTCGGGGAGTATCCT17030.1743381956252681No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16540.16932200561608537No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16290.16676272499915545No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16130.16512478540432027No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15960.1633844745848079No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCAGCTTGGTCCCTCCGCCGAACA15560.15928962559771997No Hit
GCACAGGGTCCTGGGCCCAGTTTGTCCTGACGCAGCCGCCCTCAGTGTCT15410.15775405722756203No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGACTACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG15340.15703745865482163No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14700.15048570027548097No Hit
GTCTGGCACCTCGGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAAGATG14390.14731219231048784No Hit
GTGATGGCCAGGGAGGCCGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14230.14567425271565265No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14150.14485528291823507No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14070.1440363131208175No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC13830.14157940372856473No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGACTAC13800.14127229005453315No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13730.14055569148179275No Hit
TCACTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCCAGTCCTAT13650.13973672168437518No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13470.13789403964018562No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13310.13625610004535046No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13210.13523238779857846No Hit
ATATTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGGCCTCCCTGG12730.130318569014073No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12610.1290901143179466No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12520.12816877329585183No Hit
CTACCACTCAGGCCGCTGTCATAGGACTGGCAATAATAATCAGCCTCATC12460.12755454594778862No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12400.12694031859972543No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12190.12479052288150427No Hit
ACCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGATTCCAGGAACAGCCCCCAAACTCCTCAT11780.12059330266973917No Hit
TTCCAGGAACAGCCCCCAAACTCCTCATCTATATTAACAACAATCGGCCC11710.1198767040969988No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11690.11967196164764439No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC11650.11926247674893559No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11560.11834113572684081No Hit
ATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11320.11588422633458807No Hit
GGGTCCTGGGCCCAGTTTGTCCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC11170.11434865796443008No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11130.11393917306572131No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11110.1137344306163669No Hit
GCCCCCAAACTCCTCATCTATATTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT10990.11250597592024053No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10980.11240360469556333No Hit
CTCCTGTGCAGCCTCCGGATTCATCTTTCATGATCATCTCATGCACTGGGTCCGTCAAGTTACGGGGAAGGGT10970.11230123347088614No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10840.11097040755008256No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10730.1098443240786334No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT10660.109127725505893No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10240.10482813406945067No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCGG10210.10452102039541909No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10150.1039067930473559No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10080.10319019447461553No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10070.10308782324993833No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG10050.10288308080058392No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9980.10216648222784355No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGGAGAGCAGTTGGTGGAGT9820.10052854263300837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)