FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735632
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25130.34161102290275575No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24630.33481414620353656No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22480.3055875763968941No Hit
CCTATGTGCTGACTCAGCCACCCTCTGTGTCAGTGGCCCCAGGACAGACG22290.3030047632511908No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21270.2891391347847837No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21050.28614850903712724No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20070.27282663070665764No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18640.25338756334689083No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17880.24305631076407772No Hit
GCCTTGGGCTGACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCACTACTTCTATCCCACAC16130.21926724231681058No Hit
CCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACT15550.21138286534571632No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14120.1919437979859495No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13920.18922504730626183No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13820.18786567196641799No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13810.18772973443243363No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCA13770.18718598429649608No Hit
GACCATAAGCAGGGTCGAGGTCGGGGATGAGGCCGACTACTATTGTCAGG13590.18473910868477716No Hit
GGATAGAAGTAGTGAGTTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCAGTCAGCCCAAGGCCAACCC13150.1787578571894643No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12450.16924222981055745No Hit
GATGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTGCTGGTCGT12080.16421254105313526No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12020.16339691584922897No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACT11940.1623094155773539No Hit
GCTTATGGTCAGGGTGGCCGTCTTCCCAGAGTTGGAGCCAGAGAATCGCT11550.15700785175196294No Hit
GACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCACTA11530.15673597668399417No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11350.15428910107227528No Hit
ATGATAATAGGGCCCGGCCGTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAGACGGCCACCCTGA11290.15347347586836896No Hit
CTTCTATCCCACACCTGACAATAGTAGTCGGCCTCATCCCCGACCTCGACCCTGCTTATGGTCAGGGTGGCCGT11270.15320160080040018No Hit
CTATTATCATAGACGACCAGCAGAGGGGCCTGGCCTGGCTTCTGCTGGTA11190.15211410052852514No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11060.15034691258672817No Hit
GTGTACATCTTTAGTTCCAAGATTGTTGCCCCCACAGGCAATGCTGGCCG10790.1466765991691498No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACTCAGC10380.14110316027579006No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10300.140015660003915No Hit
GCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT10260.13947190986797747No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10080.13702503425625856No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGA10060.13675315918828979No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9860.13403440850860213No Hit
CCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACTCAGCCAC9750.13253909563477392No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9720.13213128303282076No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG9540.12968440742110185No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9190.12492659373164842No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9170.12465471866367966No Hit
CTACTATTGTCAGGTGTGGGATAGAAGTAGTGAGTTTTATGTCTTCGGAA9160.12451878112969528No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9080.12343128085782022No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8850.12030471757617939No Hit
ATGTAGACTCTGTGCAGGGCCGATTCACCGTCTCCAGAGACAACGGCAAG8840.12016878004219501No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8720.11853752963438241No Hit
TGATAATAGGGCCCGGCCGTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAGACGGCCACCCTGAC8690.11812971703242926No Hit
TCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8660.1177219044304761No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8580.11663440415860105No Hit
CAATAATAGAGGCCCGCGTCTTCGGGTCTCAGACTGTCCATTTGCAGATG8520.11581877895469474No Hit
CCCTCTGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTA8510.11568284142071035No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8330.11323596580899145No Hit
GTTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT8300.1128281532070383No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA8280.11255627813906953No Hit
CTATTATTGTGCGAGAAGACGCGCGGGGGGGAATTCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC8040.10929377732344432No Hit
CTGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTACA8030.10915783978945993No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7920.10766252691563173No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC7890.10725471431367857No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT7790.10589533897383474No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7790.10589533897383474No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7650.10399221349805336No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC7620.10358440089610023No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG7610.10344846336211584No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7600.10331252582813145No Hit
GGCCAGGCCCCTCTGCTGGTCGTCTATGATAATAGGGCCCGGCCGTCAGG7560.10276877569219392No Hit
GCAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACATCTTTAGTT7560.10276877569219392No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7540.10249690062422516No Hit
GTCTGAGACCCGAAGACGCGGGCCTCTATTATTGTGCGAGAAGACGCGCG7530.10236096309024077No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7420.10086565021641256No Hit
CGGCCACCCTGACCATAAGCAGGGTCGAGGTCGGGGATGAGGCCGACTAC7400.1005937751484438No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG7400.1005937751484438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)