FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735632
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23790.32339539334884837No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23700.3221719555429889No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22200.30178132544533137No Hit
CCTATGTGCTGACTCAGCCACCCTCTGTGTCAGTGGCCCCAGGACAGACG20770.2823422580855645No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20750.28207038301759574No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19780.2688844422211106No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19630.2668453792113448No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19620.26670944167736044No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15970.21709224177306044No Hit
GCCTTGGGCTGACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCACTACTTCTATCCCACAC15880.21586880396720098No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14970.2034984883746221No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14200.19303129825782456No Hit
CCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACT14140.19221567305391826No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCA13190.17930160732540182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13000.17671879417969855No Hit
GACCATAAGCAGGGTCGAGGTCGGGGATGAGGCCGACTACTATTGTCAGG13000.17671879417969855No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12990.17658285664571416No Hit
GATGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTGCTGGTCGT12610.1714172303543076No Hit
GGATAGAAGTAGTGAGTTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCAGTCAGCCCAAGGCCAACCC12540.1704656676164169No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12430.16897035474258867No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11910.16190160297540074No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACT11400.1549687887421972No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACTCAGC11380.15469691367422841No Hit
CTTCTATCCCACACCTGACAATAGTAGTCGGCCTCATCCCCGACCTCGACCCTGCTTATGGTCAGGGTGGCCGT11330.1540172260043065No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG11320.15388128847032212No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11300.15360941340235335No Hit
GTGTACATCTTTAGTTCCAAGATTGTTGCCCCCACAGGCAATGCTGGCCG10950.14885159971289993No Hit
ATGATAATAGGGCCCGGCCGTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAGACGGCCACCCTGA10820.14708441177110296No Hit
GACTGAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAAACTCACTA10790.1466765991691498No Hit
CTATTATCATAGACGACCAGCAGAGGGGCCTGGCCTGGCTTCTGCTGGTA10660.14490941122735282No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10660.14490941122735282No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10460.14219066054766513No Hit
GCTTATGGTCAGGGTGGCCGTCTTCCCAGAGTTGGAGCCAGAGAATCGCT10100.13729690932422733No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA10090.13716097179024295No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9930.1349859712464928No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9800.13321878330469583No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGA9490.12900471975117994No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9290.12628596907149225No Hit
GCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT9180.12479065619766405No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8850.12030471757617939No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG8690.11812971703242926No Hit
CCTCTCTCACTGCACAGGCTCTGCGACCTCCTATGTGCTGACTCAGCCAC8650.11758596689649173No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8600.11690627922656982No Hit
GGGTGGCCGTCTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGACGGCCGGGCCCTATTATCATAGA8470.11513909128477282No Hit
CTACTATTGTCAGGTGTGGGATAGAAGTAGTGAGTTTTATGTCTTCGGAA8400.11418752854688215No Hit
ATGTAGACTCTGTGCAGGGCCGATTCACCGTCTCCAGAGACAACGGCAAG8370.11377971594492897No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8360.11364377841094461No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTCCTA8330.11323596580899145No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8180.1111969027992257No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT8170.11106096526524133No Hit
CTATTATTGTGCGAGAAGACGCGCGGGGGGGAATTCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC8130.11051721512930378No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8090.10997346499336624No Hit
CCCTCTGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTA8060.1095656523914131No Hit
TGATAATAGGGCCCGGCCGTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAGACGGCCACCCTGAC8060.1095656523914131No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8030.10915783978945993No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8010.10888596472149119No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7910.10752658938164734No Hit
GGCTCTGCGACCTCCTATGTGCTGACTCAGCCACCCTCTGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTGC7900.10739065184766296No Hit
CTGTGTCAGTGGCCCCAGGACAGACGGCCAGCATTGCCTGTGGGGGCAACAATCTTGGAACTAAAGATGTACA7810.10616721404180351No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC7720.10494377623594406No Hit
GTTTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT7720.10494377623594406No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC7700.10467190116797528No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7690.1045359636339909No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7670.10426408856602215No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGCGACCTC7600.10331252582813145No Hit
CAATAATAGAGGCCCGCGTCTTCGGGTCTCAGACTGTCCATTTGCAGATG7580.1030406507601627No Hit
GTGCTGACTCAGCCACCCTCTGTGTCAGTGGCCCCAGGACAGACGGCCAG7460.10140940035235008No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7370.10018596254649063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)