FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988656
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38000.38436018190351345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34850.352498745772038No Hit
GACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32690.330650903853312No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29100.294338981405059No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28720.29049537958602384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28480.2880678415950543No Hit
CTGTAAGTCTGTTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC28210.2853368613552136No Hit
GATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCA28160.28483112427376156No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26900.27208654982117136No Hit
GGTCAGAGCGTTGCCAGCTACTTTTTAAATTGGTATCAGCAGAAACCAGG26090.2638936091016491No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGA25970.2626798401061643No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24190.24467560000647343No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23690.23961822919195352No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21680.21928759851758345No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21640.2188830088524219No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21310.21554514411483874No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGAGA20850.2108923629654804No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20740.20977974138628605No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTGCCAG20500.2073522033953165No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20350.2058349921509605No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19880.20108106358531175No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19870.2009799161690214No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19820.2004741790875694No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19560.19784434626401903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19390.19612484018708226No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19280.19501221860788787No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTGCCAG19250.19470877635901668No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACATCCAGATGACCCAGTC19160.1937984496124031No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGA18880.19096632195627197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18250.18459403472997685No Hit
ACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18210.18418944506481527No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17000.17195060769367707No Hit
CCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG16130.16315078247641243No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGACCTGC15900.16082439190173325No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15440.15617161075237493No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTC15320.15495784175689015No Hit
GTCCACGGGAGACTGTAAGTCTGTTGACAGTGGTAAGTTGCAAAATCTTC15270.15445210467543818No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15210.15384522017769578No Hit
GTCCCAGACACACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT14820.14990047094237025No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14670.14838325969801427No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14600.14767522778398148No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACAT14540.14706834328623908No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14260.14423621563010794No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13840.13998802414591122No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTG13570.13725704390607046No Hit
GTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC13550.13705474907348966No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13390.13543639041284328No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTT13390.13543639041284328No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13150.13300885242187374No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13100.13250311534042175No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12940.13088475667977537No Hit
GTTCAGTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA12890.13037901959832338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12810.1295698402680002No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12770.1291652506028386No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12670.1281537764399346No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12640.12785033419106343No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12510.12653541777928823No Hit
GTGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACACACTGCCA12510.12653541777928823No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12450.12592853328154585No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTT12110.12248952112767231No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12110.12248952112767231No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12050.12188263662992992No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12040.12178148921363954No Hit
CATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG11930.12066886763444513No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11860.11996083572041236No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11780.11915165639008918No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11780.11915165639008918No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG11780.11915165639008918No Hit
GAGTGAAATCTGTCCCAGACACACTGCCACTGAACCTTGATGGGACCCCA11660.1179378873946044No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11570.1170275606479908No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGA11520.11652182356653883No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGAGACTGTAAGTCTGTTGACAG11510.11642067615024843No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11360.11490346490589244No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11280.11409428557556925No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCATCCTCC11260.11389199074298846No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11130.11257707433121329No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGA11070.1119701898334709No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10950.11075642083798612No Hit
GGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGACCTGCCCGGCAAGTGATGGTGACT10900.11025068375653412No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10840.10964379925879172No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10830.10954265184250132No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10830.10954265184250132No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10800.10923920959363013No Hit
GTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCA10780.10903691476104933No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10650.10772199834927416No Hit
GCTCTGACCTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT10590.10711511385153177No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGG10530.10650822935378938No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10430.10549675519088542No Hit
CACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCATAGATCAGGAGGTTAGGGGCTTTCC10190.10306921719991585No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10180.10296806978362545No Hit
GCCCCTAACCTCCTGATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGT10100.10215889045330226No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10090.10205774303701186No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10080.10195659562072146No Hit
ATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCACTCTCA10050.10165315337185027No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9970.10084397404152709No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9970.10084397404152709No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCACT9920.1003382369600751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)