FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988656
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47630.48176514379116697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38420.38860837338771015No Hit
GACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32230.3259981227039536No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30560.30910650418345714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30150.3049594601155508No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26990.27299687656778493No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26910.2721876972374618No Hit
CTGTAAGTCTGTTGACAGTGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC26630.2693555695813306No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26300.2660177048437475No Hit
GGTCAGAGCGTTGCCAGCTACTTTTTAAATTGGTATCAGCAGAAACCAGG26130.2642981987668107No Hit
GATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCA25540.2583305012056772No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24970.2525650984771245No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGA23760.24032626110598632No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23200.23466200579372398No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22040.22292890550403782No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22040.22292890550403782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21470.21716350277548507No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21380.21625317602887154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20510.20745335081160687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20420.20654302406499328No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGGAGA20170.20401433865773333No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGA19970.20199139033192534No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19930.2015868006667638No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19150.1936973021961127No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTGCCAG18940.19157320645401435No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGAGCGTTGCCAG18790.19005599520965838No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACATCCAGATGACCCAGTC18350.18560550889288085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17930.1813573174086841No Hit
ACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17920.18125616999239372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17610.17812060008739136No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17480.1768056836756162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16560.16750012137689954No Hit
GTCCCAGACACACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT15820.16001521257141008No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15690.15870029615963488No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGACCTGC15510.15687964266640772No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15420.15596931591979415No Hit
CCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG15030.15202456668446862No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14970.15141768218672622No Hit
GTGGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGACACACTGCCA14840.15010276577495105No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTC14830.15000161835866066No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14810.14979932352607986No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14670.14838325969801427No Hit
GTCCACGGGAGACTGTAAGTCTGTTGACAGTGGTAAGTTGCAAAATCTTC14630.14797867003285267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14230.14393277338123675No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13900.1405949086436536No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAAAGTAGCTG13890.1404937612273632No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13710.13867310773413602No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACAT13590.13745933873865127No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13240.13391917916848733No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCACT13150.13300885242187374No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGA12910.13058131443090418No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12900.1304801670146138No Hit
GCTCTGACCTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT12820.12967098768429058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12740.1288618083539674No Hit
GTCAACAGACTTACAGTCTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCAC12610.12754689194219224No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCACTGTCAACAGACTTACAGTCTCCCGTGGACGTT12530.12673771261186906No Hit
GTTCAGTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA12390.12532164878380347No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12380.12522050136751306No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12240.12380443753944748No Hit
GAGTGAAATCTGTCCCAGACACACTGCCACTGAACCTTGATGGGACCCCA12100.12238837371138192No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGA12090.12228722629509152No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12000.12137689954847793No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12000.12137689954847793No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT11990.12127575213218753No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG11970.12107345729960675No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11770.11905050897379878No Hit
GTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACCA11640.1177355925620236No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11500.11631952873395802No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11500.11631952873395802No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11380.11510575973847324No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTT11370.11500461232218284No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.11409428557556925No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11210.11338625366153648No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGTTGTGACATCCAGATGACCCAGTCTCCATCCTCC11190.11318395882895568No Hit
GGTTTCTGCTGATACCAATTTAAAAAGTAGCTGGCAACGCTCTGACCTGCCCGGCAAGTGATGGTGACT10780.10903691476104933No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGAGACTGTAAGTCTGTTGACAG10720.10843003026330694No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10710.10832888284701654No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10710.10832888284701654No Hit
CATCAAGGTTCAGTGGCAGTGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG10610.10731740868411258No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10600.10721626126782217No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10500.1062047871049182No Hit
CACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGTATGCAGCATAGATCAGGAGGTTAGGGGCTTTCC10410.1052944603583046No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGACACACTGCCACTGAACCT10380.10499101810943343No Hit
CTCCTGATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTT10190.10306921719991585No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10170.10286692236733505No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10150.10266462753475425No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10130.10246233270217345No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10000.10114741629039826No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9980.10094512145781749No Hit
GCCCCTAACCTCCTGATCTATGCTGCATACAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTGT9970.10084397404152709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATCT302.365031E-456.1217668
TATATAG200.00590331454.7149454
TCTATAC350.001694891137.6316573
GTATCAA34750.033.4674841
TGGCGAC400.003246089232.96272319
TCTAAGC653.1923253E-530.3948023
TTATACG500.005307904429.81121359
TATCAAC40050.029.352772
ACTATCG450.00580497729.27054811
ATTCACT2700.029.2705482
GATTCAC2750.028.7529031
AATGGTA2552.7030183E-928.72180470
ATCAACG41200.028.2922483
TCAACGC41450.028.2010464
AACGCAG42350.027.8363956
CAACGCA42100.027.6902265
CGCAGAG42750.027.4989098
ATGGCAA1906.601351E-626.98338170
AGAGTAC43450.026.8285311
GCAGAGT43900.026.702199
Produced by FastQC (version 0.11.5)