FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559829
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76370.48960495028621726No Hit
CTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACG75220.48223234726370645No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71910.46101207247717535No Hit
GCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT58990.37818248025905404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55380.3550389177275201No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTT54790.35125645182901455No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53210.34112713637199976No Hit
GGCGTAGACTTTGTGTTTCTCGTAGGCTGCTTTGCTCAGCGTCAGGGTGC52700.337857547205495No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49760.31900932730446735No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45130.28932658643992387No Hit
ATATTGTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAG44070.2825309697409139No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43020.27579946263340405No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40760.2613106949543828No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40670.26073370863088197No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40250.25804110578787803No Hit
GTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAAAATCAGCAGAG39250.25163014663786865No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38720.24823233828836366No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAAGG37790.24227014627885493No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37370.23957754343585097No Hit
CCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG37070.23765425569084817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36930.23675672140984685No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36420.23348713224334208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35270.22611452922083128No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCC34590.22175507699882485No Hit
GTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG34490.22111398108382394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33920.21745973436831859No Hit
GATCAGGACTCCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT33230.2130361725548121No Hit
GTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA32960.21130521358430956No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCC32880.2107923368523088No Hit
GGACTCCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGG32790.21021535052880794No Hit
GTGTAAAATCTGTGCCTGAGCCACTGCCACTGAACCTGTCAGTGACCCCG32710.20970247379680723No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGGCTGCACCA31860.20425315851929923No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31410.201368226901795No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT31400.20130411731029493No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGA31210.20008603507179315No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGTACAATATCC30490.19547014448378636No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGA30340.19450850061128497No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTTCATTTTAATGG29520.18925151410827726No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29050.18623836330777285No Hit
CAGTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA28740.18425096597126991No Hit
CTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACT28340.18168658231126616No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGGCTGCTTTGCTCAGCGTCAGGGTGCTG27750.17790411641276063No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27720.17771178763826034No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAGGCTCTGA27210.17444219847175557No Hit
GGACACGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC26930.17264712990975292No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26070.16713370504074485No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC26050.16700548585774466No Hit
TTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCTCCACTC25880.16591562280224306No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACTCTCC25740.16501808852124175No Hit
GTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT25430.16303069118473884No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25350.16251781445273808No Hit
CCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG25270.16200493772073735No Hit
TTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA25160.1612997322142363No Hit
GGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCA24930.15982521160973415No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24170.154952882655727No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24060.15424767714922596No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23600.15129863594022167No Hit
AGTCAGAGCCTCCTTCATTTTAATGGCAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA23560.15104219757422127No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23430.15020877288472007No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22940.14706740290121545No Hit
GAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22920.14693918371821527No Hit
CGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT22890.14674685494371498No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22730.14572110147971348No Hit
GAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGC22620.14501589597321246No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCT22570.144695348015712No Hit
GTCACTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA22200.1423232931302085No Hit
CTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTTCAGTGGCAGT22060.1414257588492072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22030.1412334300747069No Hit
CCACTGAACCTGTCAGTGACCCCGGAGGCCCGACTAGAACCCAAAGAGATCAGGAGTTGTGGAGACTGCCCTGG22000.14104110130020664No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21940.14065644375120606No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT21900.14040000538520567No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21880.1402717862022055No Hit
GTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACGAA21660.1388613751892034No Hit
GCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGGCTGCACCAT21610.13854082723170297No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACT21560.1382202792742025No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21150.13559178602269864No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21110.13533534765669825No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20910.13405315582669639No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20790.13328384072869526No Hit
GTCAGTGACCCCGGAGGCCCGACTAGAACCCAAAGAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA20770.13315562154569507No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20600.13206575849019347No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTCCTCACA20550.131745210532693No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20510.13148877216669264No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20370.1305912378856913No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTT20110.1289243885066889No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19900.1275780870851869No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGA19860.12732164871918653No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19750.12661644321268548No Hit
CTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTC19740.1265523336211854No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19660.12603945688918466No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT19650.12597534729768456No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19490.12494959383368305No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19470.12482137465068285No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19350.12405205955268173No Hit
GGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAG19260.1234750732291809No Hit
GAGTGGAGGCTGAAGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA19070.12225699099067912No Hit
CCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19010.12187233344167854No Hit
CTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGC18990.12174411425867836No Hit
CTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTT18610.11930794978167478No Hit
GTACAATATCCCCACTGGATCCAGAGACCCAGAGCATTAGCAGCCCCAGG18390.11789753876867272No Hit
CCTGCAGGTCGAGTCAGAGCCTCCTTCATTTTAATGGCAAGAACTATTTG18110.11610247020667008No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18020.11552548388316923No Hit
CCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGA17950.11507671674266859No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17950.11507671674266859No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17640.11308931940616568No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGCGTTTATTACTGCATGCAAGCTCTA17560.11257644267416492No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG17390.11148657961866333No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17270.1107172645206622No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17180.11014027819716135No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTC17170.11007616860566126No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17070.10943507269066033No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16940.1086016480011591No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16930.108537538409659No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGT16610.10648603148165599No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16390.10507562046865393No Hit
CCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16270.1043063053706528No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16250.10417808618765263No Hit
GTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC16200.10385753823015215No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16070.10302411354065093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)