FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559829
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78330.5021704302202357No Hit
CTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACG67500.43273974262563397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60170.3857474120560651No Hit
GCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT59170.3793364529060557No Hit
GGCGTAGACTTTGTGTTTCTCGTAGGCTGCTTTGCTCAGCGTCAGGGTGC57260.3670915209295378No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTT56500.36221919197553065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53320.3418323418785008No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48300.30964932694545366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48230.309200559804953No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47430.3040717924849454No Hit
ATATTGTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAG42980.2755430242674037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42270.27099124327089696No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40600.2602849414903813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37860.2427189134193556No Hit
GTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAAAATCAGCAGAG37650.2413726119978536No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36640.23489754325634413No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36010.2308586389918382No Hit
GTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG35800.22951233757033626No Hit
CCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG35590.22816603614883427No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35560.227973707374334No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35440.22720439227633285No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAAGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAAGG35360.2266915155443321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35090.22496055657382957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34570.2216268578158247No Hit
GGACTCCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGG34160.2189983645643208No Hit
GTGTAAAATCTGTGCCTGAGCCACTGCCACTGAACCTGTCAGTGACCCCG34140.21887014538132066No Hit
CCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33760.2164339809043171No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCC33140.21245918623131127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32990.21149754235880985No Hit
GATCAGGACTCCTCAGTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32540.2086126107413056No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32480.20822795319230505No Hit
GTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA32110.20585589830680157No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCC31630.20277863791479706No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT30310.19431617183678468No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29560.18950795247427765No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTTCATTTTAATGG29440.1887386373762765No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAGTACAATATCC28180.18066082884726467No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGGCTGCACCA28150.18046850007276438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27860.17860932191926165No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGGCTGCTTTGCTCAGCGTCAGGGTGCTG27790.178160554778761No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGA27370.17546795193575707No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGA27370.17546795193575707No Hit
GGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCA27190.17431397928875536No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27000.17309589705025358No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAGGCTCTGA26950.1727753490927531No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26880.17232658195225245No Hit
CTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACT26800.17181370522025172No Hit
CAGTAATAAACGCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGAGCCACTGCCA26700.17117260930525077No Hit
GTTCACCTCCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT26670.17098028053075048No Hit
TTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCTCCACTC26000.1666849379002442No Hit
GGACACGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATC25940.16630028035124364No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACTCTCC25670.1645693213807411No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25270.16200493772073735No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24990.1602098691587347No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACGCCAACATCTTCAGCCT24660.1580942526392316No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24490.15700438958373No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24270.15559397857072796No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23730.15213206062972287No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23610.15136274553172174No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23530.150849868799721No Hit
CCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG23520.1507857592082209No Hit
GTACTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGC23120.14822137554821715No Hit
GAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGC22930.14700329330971537No Hit
TTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA22300.14296438904520944No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22210.14238740272170858No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTCCTCACA22080.14155397803220737No Hit
CTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTTCAGTGGCAGT21750.13943836151270428No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21660.1388613751892034No Hit
AGTCAGAGCCTCCTTCATTTTAATGGCAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAA21560.1382202792742025No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21540.1380920600912023No Hit
GAGCAAAGCAGCCTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21440.13745096417620137No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21300.13655342989520006No Hit
CGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT21290.13648932030369995No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTCCACT21230.13610466275469937No Hit
GTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACGAA21230.13610466275469937No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21110.13533534765669825No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20840.13360438868619573No Hit
GCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGGCTGCACCAT20410.1308476762516917No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20150.12918082687268925No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19990.12815507340868776No Hit
CCACTGAACCTGTCAGTGACCCCGGAGGCCCGACTAGAACCCAAAGAGATCAGGAGTTGTGGAGACTGCCCTGG19830.12712931994468624No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19800.12693699117018598No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19650.12597534729768456No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19490.12494959383368305No Hit
GTCACTGACAGGTTCAGTGGCAGTGGCTCAGGCACAGATTTTACACTGAA19490.12494959383368305No Hit
GGCTTCTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAG19470.12482137465068285No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19260.1234750732291809No Hit
GTCAGTGACCCCGGAGGCCCGACTAGAACCCAAAGAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA19220.12321863486318052No Hit
CTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTT19090.12238521017367929No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGA18990.12174411425867836No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18860.12091068956917714No Hit
CTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTC18830.12071836079467686No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18440.11821808672617319No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGCCTACGAGAAACACAAAGT18250.11700000448767141No Hit
GAGTGGAGGCTGAAGATGTTGGCGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA18220.11680767571317112No Hit
GATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT18170.11648712775567066No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGACAGGTT18030.11558959347466934No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17930.1149484975596684No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTACTGACTCAGTCTC17790.11405096327866708No Hit
CTGCAGGTACCAATCCAAATAGTTCTTGCCATTAAAATGAAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGC17510.11225589471666444No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17480.11206356594216417No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17470.11199945635066409No Hit
GTACAATATCCCCACTGGATCCAGAGACCCAGAGCATTAGCAGCCCCAGG17410.11161479880166351No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17410.11161479880166351No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17280.11078137411216228No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17210.11033260697166164No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16910.10840931922665882No Hit
CCTGCAGGTCGAGTCAGAGCCTCCTTCATTTTAATGGCAAGAACTATTTG16700.10706301780515684No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16580.10629370270715571No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCA16090.10315233272365112No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16080.10308822313215102No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15690.10058794906364736No Hit
CCACAACTCCTGATCTCTTTGGGTTCTAGTCGGGCCTCCGGGGTCACTGA15630.10020329151464681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.037.9379121
GTATCAA32450.034.0709881
GATTCAC3050.033.1548461
GTGGTAT4450.032.5712171
TATCAAC34100.032.1042022
ATCAACG35000.031.3648473
TCAACGC35550.030.8786074
AACGCAG35750.030.708816
CTACGAC450.004607304530.68820841
CAACGCA35950.030.6316455
GTGCTAG4000.030.3366811
CGCAGAG36600.029.9946678
AGTGCCG803.3258511E-629.47696315
AGAGTAC37250.029.37992711
AATGGTA9750.029.27402370
ACGCAGA37950.028.9285897
CAGAGTA38300.028.66147210
AACGCTA656.165022E-428.12646361
TGGTATC5050.028.015012
ATTCACT3550.027.5170942
Produced by FastQC (version 0.11.5)