FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378711
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC78000.565745830707088No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT54500.39529676632738836No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGGGACTATTACCTCCATATGAG50110.3634554304709254No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGGGACTA46700.3387221832566796No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46500.3372715529215332No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46290.33574839106962956No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46270.33560332803611487No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43300.3140614675591912No Hit
GATTATTACTGCTGCTCATATGGAGGTAATAGTCCCCATGTGGTATTCGG41750.3028190824618067No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37820.2743141963761804No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC35800.25966282999120194No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT33920.24602690484082598No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33730.2446488060224369No Hit
GTCGATCACCATCTCCTGCACTGGAACCACCAGTGAAGTTGTAAATTATAACATTGTCTCCTGGTACCAACAACT33200.240804635634299No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32850.23826603254779286No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32830.2381209695142782No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32200.2335514839585671No Hit
GAGGTAATAGTCCCCATGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT32130.23304376334126586No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG31970.23188325907314875No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31690.22985237660394384No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31440.22803908868501088No Hit
CTATTACCTCCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTG31330.22724124200068033No Hit
CTCCTGCACTGGAACCACCAGTGAAGTTGTAAATTATAACATTGTCTCCTGGTACCAACAACTCCCAGGCAAAGT30400.2204958109422497No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT30220.21919024364061793No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30050.21795720785574352No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29360.21295253319948848No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28700.20816545309350545No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28620.20758520095944688No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27370.19851876136478203No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGAGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT27170.19706813102963566No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27100.1965604104123344No Hit
GTAAGAGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC26460.19191839333986602No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25820.1872763762673976No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCGTGGACAGTCGATCACCATCTCCTG25220.18292448526195845No Hit
GTTTGGGGACTTTGCCTGGGAGTTGTTGGTACCAGGAGACAATGTTATAA24540.17799234212246076No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24190.1754537390359546No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24030.1742932347678375No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23930.1735679196002643No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23440.17001387527915565No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23260.16870830797752395No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22470.16297831815369573No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21810.15819123804771268No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21800.15811870653095536No Hit
TTATAACATTGTCTCCTGGTACCAACAACTCCCAGGCAAAGTCCCCAAAC21000.15231618519036985No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGGGACTAT20420.14810935721844534No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18930.13730216122160482No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18880.1369395036378182No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18660.1353438102691572No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18560.13461849510158402No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17990.13048419864641683No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCGTGGACAGTCGATCAC17810.12917863134478508No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC17730.12859837921072656No Hit
CTCGTGGACAGTCGATCACCATCTCCTGCACTGGAACCACCAGTGAAGTT17590.12758293797612408No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17430.126422433708007No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17280.1253344609566472No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17270.12526192943988987No Hit
GACTCAGCCTGCCTCCGTTTCTGGGTCTCGTGGACAGTCGATCACCATCT17160.12446408275555937No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17160.12446408275555937No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17130.12424648820528741No Hit
GTGCAGGAGATGGTGATCGACTGTCCACGAGACCCAGAAACGGAGGCAGG17090.12395636213825811No Hit
CCTTGGTCCCTCCGCCGAATACCACATGGGGACTATTACCTCCATATGAGCAGCAGTAATAATCAGCCTCGTCCT17010.12337611000419958No Hit
CCCCTGAGGGCCTCTTACTGCCCTCATAAATGATGAGTTTGGGGACTTTGCCTGGGAGTTGTTGGTACCAGGAGA16800.12185294815229587No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16400.11895168748200312No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16180.1173559941133421No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16030.11626802136198232No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15980.11590536377819571No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC15800.11459979647656397No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15740.11416460737602008No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA15260.11068309457166875No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG15220.11039296850463949No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT15200.11024790547112485No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG15120.1096676533370663No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15050.10915993271976507No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14920.10821702300191992No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14800.10734664480083209No Hit
GCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCGTGGACAGTCGATCAC14700.1066213296332589No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG14590.10582348294892838No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGGGGA14450.10480804171432592No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14420.10459044716405397No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14370.10422778958026736No Hit
GTATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC14370.10422778958026736No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14360.10415525806351005No Hit
TCATTTATGAGGGCAGTAAGAGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT14260.10342994289593685No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14220.10313981682890758No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14090.10219690711106243No Hit
ATGTTATAATTTACAACTTCACTGGTGGTTCCAGTGCAGGAGATGGTGAT13830.10031108767537214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6000.067.4383670
CGTATGC5850.047.21999441
TCTCGTA5500.045.35513338
ATGCCGT6150.045.34217544
TCGTATG6150.044.76699440
ATCTCGT5650.044.0479137
CTAGCGC6550.043.96331828
ACTAGCG6550.043.92973327
GGTATCA13350.043.231881
GCCGTCT6500.043.16734746
TAGCGCT6700.043.0354729
CCGTCTT6700.042.55317347
CCCACGA6700.042.31907319
GACTAGC6800.042.27314426
CGTCTTC6450.041.6029448
TATACAC7100.041.3398673
GCGCTCA6850.041.23745331
TATGCCG6750.041.19777343
CGCTCAT6600.040.85102532
TCTCCGA6900.040.60457211
Produced by FastQC (version 0.11.5)