FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476925
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82660.55967635458808No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC80820.5472180374765138No Hit
GTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA60600.41031196573962797No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA58350.39507761057602786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55400.37510367825041896No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55400.37510367825041896No Hit
GTAAGAATACTGCTGACAGTGATAAAATGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT54960.3721245154628705No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA50790.34389017722633175No Hit
GTATTCTTACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT48990.3317026930954517No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48020.3251349933138108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46610.31558813074462144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45960.3111870948084703No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43840.2968329468321005No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42370.2868798347918818No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42070.28484858743673513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39950.27049443946036533No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37550.2542444606191919No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36230.24530697225654655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35350.23934864668144962No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34600.23427052829358294No Hit
GTCTCCAGCCACCCTGTCTGTGGTTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGAC34270.23203615620292162No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34130.23108824077051984No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC32450.21971325558169846No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC32300.2186976319041251No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG31370.21240076510317044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30610.20725493847013218No Hit
GTCTGTGGTTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTA28080.19012475244172858No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27780.18809350508658193No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGTTCCAGG27650.1872132978993517No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATGTTGCATTTTA27520.18633309071212145No Hit
GTCTTAATACTCTGACTGGCCCGGCAGGAGAGGGTGGCTCTTTCCCCTGGAACCACAGACAGGGTGGCTGGAGA27410.18558830001523435No Hit
GTCTGAAGATGTTGCATTTTATCACTGTCAGCAGTATTCTTACTGGGTCA27310.1849112175635188No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27240.1844372598473179No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27170.183963302131117No Hit
GAATACTGCTGACAGTGATAAAATGCAACATCTTCAGACTGCAGGCTGCT26390.17868205900773568No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCGCAGACCCACTGCCACTGAACCTGGCTGGGATA26290.1780049765560201No Hit
CACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACTCTCACCATCAGCAG26160.1771247693687899No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACT26090.176650811652589No Hit
TCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACT26010.17610914569121655No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT25720.17414560658124142No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25610.17340081588435433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24480.16574978417996852No Hit
GAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC24430.16541124295411075No Hit
CATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCA24140.16344770384413562No Hit
GCAGTATTCTTACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT23700.16046854105658717No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23640.16006229158555785No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23080.1562706298559507No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22780.15423938250080405No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22760.15410396601046095No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22720.1538331330297747No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22680.15356230004908847No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22450.1520050104101427No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22080.14949980533879514No Hit
GAGTGAACTCTGTCGCAGACCCACTGCCACTGAACCTGGCTGGGATACCA21780.14746855798364844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21350.14455710344127157No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21240.14381231274438444No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21220.14367689625404134No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21100.14286439731198267No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21070.142661272576468No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC20970.14198419012475244No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20690.14008835925994886No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20670.13995294276960576No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20220.13690607173688576No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20120.13622898928517022No Hit
GTCTGCGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG20010.1354841985882831No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19720.133520659478308No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC19480.1318956615941906No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19470.13182795334901906No Hit
GTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACTCTCACCATCAGCAGCC19220.1301352472197302No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19220.1301352472197302No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT18720.12674983496115239No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18500.12526025356737816No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18420.12471858760600572No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT18270.12370296392843237No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18200.1232290062122315No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC18160.12295817323154526No Hit
GCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTTGGT18110.1226196320056875No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18030.12207796604431505No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG17950.12153630008294261No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17830.12072380114088394No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17330.11733838888230615No Hit
CTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT17210.11652588994024747No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGTTCCAGGGGAAAGAGCCAC17090.11571339099818881No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17040.11537484977233103No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATGTTGCATTTTATCACTGTCAGCAGTATTCTTACTGGGTCACTTTCGG17030.11530714152715947No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16950.11476547556578702No Hit
AACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCG16740.11334360241718436No Hit
CCTCATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTT16700.11307276943649813No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16530.11192172926858168No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16420.11117693857169457No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16320.110499856119979No Hit
GAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGG15960.108062359293803No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15860.10738527684208744No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15690.10623423667417099No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT15610.10569257071279856No Hit
GTCGCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGCGGCCCTGGT15520.10508319650625454No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15490.10488007177073988No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTTGGTCTTA15450.10460923879005365No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15340.10386444809316654No Hit
GGATACCAGCGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGG15290.10352590686730875No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15250.10325507388662256No Hit
ACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATC15230.10311965739627943No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15000.10156236775733364No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14960.10129153477664743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT200.00745262451.58553339
CTCGACG3550.049.6592528
CGTATGC3350.048.4227141
ACTCGAC3700.047.60722427
TCGACGT3800.047.3197229
GTCGTAT800.047.2867439
GGTATCA10950.047.2271041
TCGTATG3300.046.9817540
GACTCGA3900.046.0078526
CGACGTC3850.045.87385630
GACGTCA3900.045.3397431
ATGCCGT3400.044.91536344
AGACTCG4150.043.2113725
ACGAGAC4150.042.36158822
CACGAGA4250.041.3620421
TCTCCGA4250.041.33543811
TGTCGTA759.222276E-1041.16345238
GCCGTCT3750.040.88965246
CCCACGA4300.040.87001419
TATACAC4700.040.255733
Produced by FastQC (version 0.11.5)