FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476925
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83570.5658378048986915No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63180.4277806929938894No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58850.39846302283460566No Hit
GTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCA58770.39792135687323327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57460.38905157675575947No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA54730.3705672258239247No Hit
GTAAGAATACTGCTGACAGTGATAAAATGCAACATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT51060.34571829984596375No Hit
GTATTCTTACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT45970.31125480305364184No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45870.3105777206019263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45400.3073954330788632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44950.3043485620461432No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42910.2905360800311458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42810.28985899757943023No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41290.2795673443133537No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37930.256817373935711No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37810.2560048749936524No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37110.2512652978316435No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35890.24300489192071364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35790.24232780946899807No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG35780.24226010122382655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34930.2365049003842443No Hit
GTCTCCAGCCACCCTGTCTGTGGTTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGAC33680.22804136973779982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33450.22648408009885404No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT32810.22215075240787446No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG30560.2069163972442744No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29790.20170286236606463No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC29330.19858828308817306No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC29220.19784349239128596No Hit
GTCTGTGGTTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTA29200.19770807590094283No Hit
GTCTTAATACTCTGACTGGCCCGGCAGGAGAGGGTGGCTCTTTCCCCTGGAACCACAGACAGGGTGGCTGGAGA28040.18985391946104238No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27670.1873487143896948No Hit
GTCTGAAGATGTTGCATTTTATCACTGTCAGCAGTATTCTTACTGGGTCA26470.1792237249691081No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATGTTGCATTTTA26300.17807268480119165No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGTTCCAGG26040.17631227042673123No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA25890.17529664674915788No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACT25700.17401019009089833No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25390.17191123449058007No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25080.16981227889026188No Hit
GAATACTGCTGACAGTGATAAAATGCAACATCTTCAGACTGCAGGCTGCT24910.16866123872234542No Hit
TCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCGCAGACCCACTGCCACTGAACCTGGCTGGGATA24900.16859353047717385No Hit
GAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC24520.16602061716065472No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT24440.1654789511992823No Hit
CACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACTCTCACCATCAGCAG24210.16392166156033652No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23440.1587081266821267No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23390.15836958545626895No Hit
CATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCA23290.1576925030045534No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22670.15349459180391692No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22600.15302063408771605No Hit
TCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACT22580.15288521759737292No Hit
GCAGTATTCTTACTGGGTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22490.15227584339082892No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21770.1474008497384769No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC21640.14652064255124667No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21620.14638522606090357No Hit
GAGTGAACTCTGTCGCAGACCCACTGCCACTGAACCTGGCTGGGATACCA21500.14557272711884492No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21490.14550501887367334No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21380.14476022817678624No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21300.1442185622154138No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21130.14306752204749734No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20850.14117169118269376No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20280.1373123212079151No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20030.1356196150786262No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19920.1348748243817391No Hit
GTCTGCGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATG19830.1342654501751951No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19800.1340623254396804No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19760.1337914924589942No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18690.12654671022563774No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18460.12498942058669195No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18140.12282275674120216No Hit
GCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTTGGT17680.1197081774633106No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT17530.11869255378573725No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17500.1184894290502226No Hit
CTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT17460.11821859606953636No Hit
GTTCAGTGGCAGTGGGTCTGCGACAGAGTTCACTCTCACCATCAGCAGCC17440.11808317957919326No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17370.11760922186299237No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17350.11747380537264925No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17200.11645818169507592No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17060.11551026626267413No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17060.11551026626267413No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17040.11537484977233103No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGC16970.11490089205613015No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16840.11402068486889991No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16730.11327589417201278No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC16710.11314047768166968No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16630.11259881172029725No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16460.1114477715523808No Hit
GAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTATTAAGACCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGG16370.11083839734583678No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTTGGTCTTA16350.11070298085549368No Hit
CCTCATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGCGACAGAGTT16230.109890481913435No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16070.10880714999069012No Hit
GTCGCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGCGGCCCTGGT16070.10880714999069012No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG15970.10813006753897456No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15700.10630194491934256No Hit
AACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCG15670.10609882018382788No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATGTTGCATTTTATCACTGTCAGCAGTATTCTTACTGGGTCACTTTCGG15640.10589569544831323No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15330.10379673984799499No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG15100.1022394502090492No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGGTTCCAGGGGAAAGAGCCAC14990.10149465951216209No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14990.10149465951216209No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14960.10129153477664743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)