FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799786
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT184181.0233438864398323No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC149670.8315988678654018No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAG118870.6604674111255449No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT113060.6281857954223447No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC93950.5220065052178426No Hit
GACCAGGACAGCCTCCGAAACTGCTCATTTACGGGGCATCTACCCGGGAA91360.5076159054465363No Hit
ATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA85910.4773345275493864No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA73150.40643720975716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG70940.39415797211446246No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC70620.3923799829535289No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC67600.3756002102472183No Hit
GAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT66780.371044113022326No Hit
GTCCTGGTCTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG60480.3360399514164462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT59620.3312616055464372No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATA58090.3227605948707235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA57550.3197602381616481No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT55870.31042579506674683No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG53560.29759093581125756No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG53300.29614631961799903No Hit
CTCCGAAACTGCTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCT50150.2786442388150591No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49320.27403257942888765No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC48290.2683096768171327No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48050.26697618494643255No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA47560.264253639043753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG47050.2614199688185151No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46730.2596419796575815No Hit
TTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTT46720.25958641749630235No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT46110.2561971256582727No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45580.25325233111047646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43970.2443068231445294No Hit
GTTTATTACTGTCAGCAGTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA43720.24291776911255003No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42050.23363888817892792No Hit
GTGCTGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC41960.2331388287274154No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAA39480.21935941273018017No Hit
GTATCAGCAGAGACCAGGACAGCCTCCGAAACTGCTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCTGA39050.21697023979517566No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38850.21585899656959215No Hit
GTTTCGGAGGCTGTCCTGGTCTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACATT38160.21202520744132916No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCTGC37930.2107472777319081No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA37140.20635786699085334No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCCGTAAATGAGCAGTTTCGGAGGCTGTCCTGGTCTCTGCTGATA36090.20052384005654006No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA35880.19935703466967739No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG35770.19874585089560648No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35390.19663448876699785No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC34650.19252288883233895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA33970.1887446618653551No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG33830.18796679160744667No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC33750.18752229431721326No Hit
GGCCAAAAGTGTACGGAGTACTATAATACTGCTGACAGTAATAAACTGCC33370.18541093218860466No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32850.18252169980208757No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACAATAAGAACTA32110.17841009986742867No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTACT31960.17757666744824108No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30890.17163151619136943No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30680.17046471080450676No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT30470.16929790541764408No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29930.1662975487085687No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCC29730.16518630548298519No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA28170.156518608323434No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT28090.15607411103320062No Hit
GCCTACGGGGACATCGTGCTGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA27590.1532960029692419No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT27550.1530737543241252No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27520.15290706784028768No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA27470.1526292570338918No Hit
CTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACAT27290.15162913813086668No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT26790.14885103006690795No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26650.14807315980899952No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC26190.1455173003901575No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC25770.14318368961643216No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25740.14301700313259466No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25360.14090564100398603No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT24940.1385720302302607No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24840.13801640861746897No Hit
GCAGAGACCAGGACAGCCTCCGAAACTGCTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT24530.13629398161781456No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24450.13584948432758115No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC24410.13562723568246446No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24390.13551611135990613No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24080.1337936843602517No Hit
ATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGA23990.13329362490873914No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23930.13296025194106412No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG23900.1327935654572266No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23670.13151563574780556No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC23360.12979320874815117No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC23170.12873752768384686No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC23030.12795965742593843No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC23010.12784853310338007No Hit
CAATAAGAACTACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCGAAACTGCTCATTTACGGGGCATCTAC22990.12773740878082174No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT22920.1273484736518675No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22750.12640391691012154No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22590.12551492232965475No Hit
GAACTACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCGAAACTGC22230.12351468452360446No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22170.12318131155592943No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT21960.12201450616906677No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG21880.12157000887883337No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT21640.1202365170081332No Hit
CTACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCGAAACTGCTCATTTACGGGGCATCTACCCGGGAATC21390.11884746297615384No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21350.11862521433103715No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21240.11801403055696622No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21230.11795846839568704No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20840.11579154410579924No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC20690.11495811168661164No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTG20540.114124679267424No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20500.11390243062230732No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20400.11334680900951559No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20290.11273562523544466No Hit
ACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCGAAACTGCTCATT19920.1106798252681152No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19790.10995751717148593No Hit
GGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCA19760.10979083068764842No Hit
CTACGGGGACATCGTGCTGGCCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG19650.10917964691357751No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTACTCCGTACACTTTTGGCCAGGGGACCAAG19560.10867958746206494No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19000.10556810643043119No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCCGTAAATGAGCAGTTTCGGAGGCTGTCCTGGT18960.10534585778531448No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCTGCCTACGGGGACATCGTGCTGG18890.10495692265636025No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG18760.10423461455973099No Hit
ACCATCAACTGCAAGTCCAGCCAGAATGTTTTATACAGCTCCAACAATAA18710.10395680375333513No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18570.10317893349542667No Hit
TTATTACTGTCAGCAGTATTATAGTACTCCGTACACTTTTGGCCAGGGGA18440.10245662539879741No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGCTGCCTACGGGGACATCGTG18350.10195656594728483No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGC18270.10151206865705144No Hit
GCTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAGACCAGGACAGCCTCCGAAACTGCTCATTTACGGGG18170.10095644704425971No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC18120.10067863623786383No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18010.10006745246379292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)