FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86080
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT16791.950511152416357No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC2840.3299256505576208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.26370817843866173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.2509293680297398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.22421003717472118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.21143122676579926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.1928438661710037No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1630.18935873605947956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.17193308550185873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.16728624535315986No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.1475371747211896No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.14521375464684014No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.13940520446096655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.13940520446096655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1130.1312732342007435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1254646840148699No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.12197955390334572No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.11849442379182155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.11617100371747212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.11617100371747212No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.11036245353159851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.10571561338289963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1045539033457249No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.10106877323420074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)