FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86080
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29143.3852230483271377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.30436802973977695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.28113382899628253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.2578996282527881No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.2137546468401487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1650.191682156133829No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.1649628252788104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.16147769516728624No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.14869888475836432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1260.14637546468401488No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.13824349442379183No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.1359200743494424No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.11965613382899627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.11733271375464684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT960.11152416356877323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.11152416356877323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.10920074349442378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.10920074349442378No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.10803903345724906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.10571561338289963No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG900.1045539033457249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.10339219330855018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTG150.004065766560.07355522
GGGTATG150.004113007359.898721
GGTATGA150.004113007359.898722
TATAGGA150.00412250459.8638737
GACCTTT150.00412250459.8638736
AAAATGA250.007201575751.9727360
TGTAAAT250.00741998951.58195559
GCTGGAC303.780204E-450.968846
ACGGGTA300.001266951239.88604416
AGTATCA350.002685328734.227842
ACGGGAA1004.7293724E-1132.9059916
GGGAAGC551.9202233E-532.6910618
TTAGCCT400.003394076132.6271433
TTTAGCC450.00632531528.72864232
ATTTAGC450.00668280728.40745231
TAGCCTT500.00942921526.47099934
GTATCAA15150.026.2921771
CGGGAAG1152.1792039E-723.42499417
TATCAAC17300.022.8515342
GCAGAGT16750.022.1585679
Produced by FastQC (version 0.11.5)