FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196998
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58710.49047700998665No Hit
GTTAGGACCCAGAGGGAGCCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA47480.3966589752029661No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43670.36482934808579465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42820.3577282501725149No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT41650.34795379775070634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41410.3459487818693097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38070.31804564418654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35960.30041821289592796No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTGTT35650.29782840071579064No Hit
GTCTCCGGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGC35250.2944867075801296No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34670.2896412525334211No Hit
GTGTATGGCCAGTTGCTACGCAGCTGACAGTAATAAACTGCAAAATCTTC34380.28721852501006684No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33510.27995034244000405No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGCCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT30570.2553888978928954No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG29450.24603215711304446No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29140.24344234493290715No Hit
GTTTATTACTGTCAGCTGCGTAGCAACTGGCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAA29110.2431917179477326No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28810.24068544809598677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28710.23985002481207154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28010.2340020618246647No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCTGCGTAGCAACTGG27640.23091099567417822No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27420.22907306444956466No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27360.2285718104792155No Hit
GTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26300.21971632366971375No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGCCTACTTAGCCTGGTA26210.21896444271419002No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25930.2166252575192273No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25560.2135341913687408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25430.21244814109965096No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA25340.21169626014412724No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24760.20685080509741874No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCC24680.2061824664702865No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23630.19741052198917627No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21840.18245644520709306No Hit
GCTCAGTTAGGACCCAGAGGGAGCCATGGAAGCCCCAGCTCAGCTTCTCT21410.17886412508625746No Hit
GTCAGAGTGTTAGCGCCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG20950.17502117798024727No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT20400.1704263499187133No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC20250.1691732149928404No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19910.16633277582752853No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19530.16315816734865055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19170.16015064352655559No Hit
CAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG18980.1585633392871166No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18660.15588998477858776No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTT18440.1540520535539742No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCA18240.15238120698614369No Hit
GTGTTGACACAGTCTCCGGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGC18210.1521305800009691No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18070.15096098740348773No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17790.148621802208525No Hit
GAGTGTTAGCGCCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC17680.1477028365962182No Hit
GCGTAGCAACTGGCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT17610.14711804029747752No Hit
CATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17150.14327509319146733No Hit
GCCCTGTTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGTAGGCG17110.14294092387790122No Hit
GTGTTAGCGCCTACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC17110.14294092387790122No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16920.14135361963846224No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16880.14101945032489613No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16880.14101945032489613No Hit
TACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCG16770.14010048471258932No Hit
GGCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16750.1399334000558063No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16300.1361739952781876No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15820.13216396351539433No Hit
CTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACAAAGACAGGGTGGCCGG15820.13216396351539433No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15770.13174625187343672No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15690.1310779132463045No Hit
CTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCA15100.12614891587120447No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15090.12606537354281294No Hit
CACCATCAGCAGCCTGGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCTGCGTAGCAACTGGCCATACACTTT15020.12548057724407227No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14950.12489578094533157No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14840.12397681533302478No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC14750.12322493437750105No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14710.12289076506393495No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14580.12180471479484511No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14510.12121991849610443No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14510.12121991849610443No Hit
GGCCGAAAGTGTATGGCCAGTTGCTACGCAGCTGACAGTAATAAACTGCA14360.11996678357023152No Hit
CAGTTAGGACCCAGAGGGAGCCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14320.11963261425666542No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14100.11779468303205186No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC14090.11771114070366032No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGC14010.1170428020765281No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13910.11620737879261284No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13800.11528841318030607No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13680.11428590523960774No Hit
GTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGC13580.11345048195569249No Hit
GGCTAAGTAGGCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTC13570.11336693962730096No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCGGCCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCAC13540.11311631264212638No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT13520.11294922798534333No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13520.11294922798534333No Hit
GCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGG13400.11194672004464501No Hit
GCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGTATGG13160.1099417041632484No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13150.10985816183485687No Hit
GCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATC13060.10910628087933315No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13010.1086885692373755No Hit
GCCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGCTAAGTAGGCGCTA12890.1076860612966772No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12890.1076860612966772No Hit
GGCTTCTGTTGGTACCAGGCTAAGTAGGCGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTC12850.10735189198311108No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGG12830.10718480732632803No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12830.10718480732632803No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12650.10568104541528056No Hit
CACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACAAA12560.10492916445975682No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12540.10476207980297378No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12500.10442791048940767No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12380.10342540254870937No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12200.10192164063766189No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12060.10075204804018052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)